論文 - 詳細
| RRC ID | 30025 |
|---|---|
| 著者 | Way JC, Davis MA, Morisato D, Roberts DE, Kleckner N. |
| タイトル | New Tn10 derivatives for transposon mutagenesis and for construction of lacZ operon fusions by transposition. |
| ジャーナル | Gene |
| Abstract |
We describe below several new variants of the tetracycline-resistance transposon Tn10 which are more useful than the wild-type transposon for many types of genetic and physical analysis of bacteria. These derivatives have one or more of the following new properties: (i) new drug resistance markers; (ii) high transposition frequencies; (iii) removal of the transposase gene to a position outside of the transposing segment; (iv) internal deletions which eliminate the ability of Tn10 to make adjacent deletion/inversions; or (v) addition of a trp-lac operon fusion segment just inside one terminus such that insertion can automatically generate a transcriptional fusion to the interrupted operon. Phage and plasmid vehicles carrying these new elements are described. |
| 巻・号 | 32(3) |
| ページ | 369-79 |
| 公開日 | 1984-12-1 |
| DOI | 10.1016/0378-1119(84)90012-x |
| PII | 0378-1119(84)90012-X |
| PMID | 6099322 |
| MeSH | DNA Transposable Elements* Escherichia coli / genetics* Genetic Engineering / methods* Genetic Vectors Lac Operon* Mutation* Plasmids beta-Galactosidase / genetics |
| IF | 2.984 |
| 引用数 | 593 |
| WOS 分野 | GENETICS & HEREDITY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 20 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 原核生物(大腸菌) | ME9018 |