Reference - Detail
| RRC ID | 30065 |
|---|---|
| Author | Ogawa T, Pickett GG, Kogoma T, Kornberg A. |
| Title | RNase H confers specificity in the dnaA-dependent initiation of replication at the unique origin of the Escherichia coli chromosome in vivo and in vitro. |
| Journal | Proc Natl Acad Sci U S A |
| Abstract |
Escherichia coli rnh mutants defective in RNase H activity display the features of previously described sdrA (stable DNA replication) and dasF (dnaA suppressor) mutants: (i) sustained DNA replication in the absence of protein synthesis, (ii) lack of requirement for dnaA protein and the origin of replication (oriC), and (iii) sensitivity of growth to a rich medium. Both the sdrA mutants (selected for continued DNA replication in the absence of protein synthesis) and the dasF mutants (selected as dnaA suppressors) are defective in RNase H activity, measured in vitro. Furthermore, a 760-base-pair fragment containing the rnh+ structural gene complements the phenotype of each of the rnh, sdrA, and dasF mutants, indicative of a single gene. One function of RNase H in vivo is in the initiation of a cycle of DNA replication at oriC dependent on dnaA+. In keeping with these results, RNase H contributes to the specificity of dnaA protein-dependent replication initiated at oriC in a partially purified enzyme system. |
| Volume | 81(4) |
| Pages | 1040-4 |
| Published | 1984-2-1 |
| DOI | 10.1073/pnas.81.4.1040 |
| PMID | 6322184 |
| PMC | PMC344759 |
| MeSH | Bacterial Proteins / genetics* Chloramphenicol / pharmacology Chromosomes, Bacterial / physiology* DNA Replication* Endoribonucleases / metabolism* Escherichia coli / drug effects Escherichia coli / genetics* Genes* Genes, Bacterial* Genetic Complementation Test Genotype Mutation* Phenotype Ribonuclease H Species Specificity Suppression, Genetic |
| IF | 9.412 |
| Times Cited | 128 |
| WOS Category | BIOLOGY |
| Altmetric score |
オルトメトリクス指標項目
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| The most frequently cited source | Patent(IFI CLAIMS) |
| Total number of mentions | 1 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| Prokaryotes E. coli | ME9177 ME9178 ME9179 ME9136 ME9183 ME9166 ME9167 ME9132 |