| RRC ID |
30116
|
| 著者 |
Hong X, Cadwell GW, Kogoma T.
|
| タイトル |
Escherichia coli RecG and RecA proteins in R-loop formation.
|
| ジャーナル |
EMBO J
|
| Abstract |
Escherichia coli rnhA mutants devoid of RNase HI exhibit constitutive stable DNA replication, cSDR, which is thought to be initiated from R-loops stabilized in the absence of RNase HI. We found that a combination of an rnhA and a recG mutation is lethal to the cell. recG mutations that inactivate the helicase activity of RecG protein and inhibit reverse branch migration of Holliday junctions impart phenotypes resembling those of rnhA mutants. Thus, recG mutants display cSDR activity, and recG polA double mutants are inviable as are rnhA polA double mutants. These results suggest that the RecG helicase has a role in preventing R-loop formation. A model that R-loops are formed by assimilation of RNA transcripts into the duplex DNA is discussed. The model further postulates that RecA protein catalyzes this assimilation reaction and that RecG protein counteracts RecA in this reaction, resolves R-loops by its helicase activity, or does both.
|
| 巻・号 |
14(10)
|
| ページ |
2385-92
|
| 公開日 |
1995-5-15
|
| DOI |
10.1002/j.1460-2075.1995.tb07233.x
|
| PMID |
7774596
|
| PMC |
PMC398347
|
| MeSH |
Bacterial Proteins / genetics
Bacterial Proteins / metabolism*
DNA Helicases / genetics
DNA Helicases / metabolism*
DNA Polymerase I / genetics
DNA Repair
DNA Replication / genetics*
DNA, Bacterial / biosynthesis
DNA-Binding Proteins / genetics
Escherichia coli / genetics*
Escherichia coli Proteins*
Genes, Bacterial / genetics
Genes, Lethal / genetics
Kanamycin Resistance / genetics
Models, Genetic
Mutation
Nucleic Acid Conformation
Nucleic Acid Heteroduplexes
Rec A Recombinases / metabolism*
Recombination, Genetic
Replication Origin
Ribonuclease H / analysis
Ribonuclease H / genetics
SOS Response, Genetics
Sequence Analysis, DNA
|
| IF |
9.889
|
| 引用数 |
75
|
|
WOS 分野
|
BIOCHEMISTRY & MOLECULAR BIOLOGY
CELL BIOLOGY
|
| リソース情報 |
| 原核生物(大腸菌) |
ME9375
ME9580
ME9582
ME9583
ME9586
ME9576
ME9578
ME9569
ME9573
ME9355 |
