Reference - Detail
|Author||Asai T, Bates DB, Kogoma T.|
|Title||DNA replication triggered by double-stranded breaks in E. coli: dependence on homologous recombination functions.|
Homologous recombination-dependent DNA replication (RDR) of a lambda cos site-carrying plasmid is demonstrated in E. coli cells when the cells express lambda terminase that introduces a double-stranded break into the cos site. RDR occurs in normal wild-type cells if the plasmid also contains the recombination hotspot chi. Chi is dispensable when cells are induced for the SOS response or contain a recD mutation. recBC sbcA mutant cells are also capable of RDR induction. A recN mutation greatly reduces RDR in normal cells, but not in SOS-induced cells. RDR proceeds by the theta mode or rolling circle mode of DNA synthesis, yielding covalently closed circular plasmid monomers or linear plasmid multimers, respectively. Previously described inducible stable DNA replication is considered to be a special type of RDR that starts exclusively from specific sites (oriMs) on the chromosome.
|MeSH||Bacterial Proteins / genetics Base Sequence DNA Damage* DNA Repair / genetics DNA Replication* DNA Restriction Enzymes* Endodeoxyribonucleases / genetics Escherichia coli / genetics* Escherichia coli Proteins* Exodeoxyribonuclease V Exodeoxyribonucleases / genetics Models, Genetic Molecular Sequence Data Mutation Plasmids / biosynthesis* Plasmids / genetics Recombination, Genetic* SOS Response (Genetics) Sequence Homology, Nucleic Acid|
|WOS Category||BIOCHEMISTRY & MOLECULAR BIOLOGY CELL BIOLOGY|
|Prokaryotes E. coli||ME9348 ME9563 ME9556 ME9557 ME9548 ME9541 ME9549|