| RRC ID |
30164
|
| Author |
Nakano H, Goto S, Nakayashiki T, Inokuchi H.
|
| Title |
Temperature-sensitive mutations in various genes of Escherichia coli K12 can be suppressed by the ssrA gene for 10Sa RNA (tmRNA).
|
| Journal |
Mol Genet Genomics
|
| Abstract |
An Escherichia coli strain with a deletion in the ssrA gene that encodes 10Sa RNA (tmRNA) was used to screen for temperature-sensitive (ts) mutants whose ts phenotypes were suppressible by introduction of the wild-type ssrA gene. Mutants in four different genes were isolated. Ts mutants of this type were also obtained in a screen for mutations in thyA, the structural gene for thymidylate synthase. The ThyA activity in crude extracts prepared from the ts mutants was temperature-sensitive. The presence of the ssrA gene caused an increase in the total amount of the temperature-sensitive enzyme expressed, rather than suppressing the ts activity of the enzyme itself. SsrA-DD, a mutant form of 10Sa RNA, suppressed the ts phenotype of a thyA mutant, suggesting that degradation of a tagged peptide was not required for suppression of the ts phenotype. Considering the fact that ssrA-suppressible mutants could be isolated as temperature-sensitive mutants with mutations in different genes, it seems evident that trans-translation can occur on mRNA that is not lacking its stop codon.
|
| Volume |
265(4)
|
| Pages |
615-21
|
| Published |
2001-6-1
|
| DOI |
10.1007/s004380100453
|
| PMID |
11459181
|
| MeSH |
Amino Acid Substitution
Bacterial Proteins / genetics
Codon, Terminator
Escherichia coli / genetics*
Gene Expression Regulation, Bacterial*
Genes, Bacterial*
Genetic Complementation Test
Mutation*
Phenotype
Point Mutation
Protein Biosynthesis
RNA, Bacterial / genetics
RNA, Bacterial / physiology*
Ribose-Phosphate Pyrophosphokinase / genetics
Sequence Deletion
Suppression, Genetic*
Temperature
Thymidylate Synthase / genetics
|
| IF |
2.797
|
| Times Cited |
14
|
|
WOS Category
|
GENETICS & HEREDITY
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
| Resource |
| Prokaryotes E. coli |
IH39
IH40
IH41
IH42 |
