RRC ID 30205
Author Li C, Wang A, Ma X, Pourkheirandish M, Sakuma S, Wang N, Ning S, Nevo E, Nawrath C, Komatsuda T, Chen G.
Title An eceriferum locus, cer-zv, is associated with a defect in cutin responsible for water retention in barley (Hordeum vulgare) leaves.
Journal Theor. Appl. Genet.
Abstract Drought limits plant growth and threatens crop productivity. A barley (Hordeum vulgare) ethylene imine-induced monogenic recessive mutant cer-zv, which is sensitive to drought, was characterized and genetically mapped in the present study. Detached leaves of cer-zv lost 34.2 % of their initial weight after 1 h of dehydration. The transpiration was much higher in cer-zv leaves than in wild-type leaves under both light and dark conditions. The stomata of cer-zv leaves functioned normally, but the cuticle of cer-zv leaves showed increased permeability to ethanol and toluidine blue dye. There was a 50-90 % reduction in four major cutin monomers, but no reduction in wax loads was found in the cer-zv mutant as compared with the wild type. Two F(2) mapping populations were established by the crosses of 23-19 × cer-zv and cer-zv × OUH602. More polymorphisms were found in EST sequences between cer-zv and OUH602 than between cer-zv and 23-19. cer-zv was located in a pericentromeric region on chromosome 4H in a 10.8 cM interval in the 23-19 × cer-zv map based on 186 gametes tested and a 1.7 cM interval in the cer-zv × OUH602 map based on 176 gametes tested. It co-segregated with EST marker AK251484 in both maps. The results indicated that the cer-zv mutant is defective in cutin, which might be responsible for the increased transpiration rate and drought sensitivity, and that the F(2) of cer-zv × OUH602 might better facilitate high resolution mapping of cer-zv.
Volume 126(3)
Pages 637-46
Published 2013-3
DOI 10.1007/s00122-012-2007-3
PMID 23124432
MeSH Chlorophyll / metabolism Chromosome Mapping Chromosomes, Plant DNA, Plant / genetics Expressed Sequence Tags Genes, Plant* Genetic Linkage Genetic Loci* Genetic Markers Genotype Hordeum / genetics* Hordeum / growth & development Membrane Lipids / metabolism* Mutation Plant Leaves / genetics* Plant Leaves / growth & development Water / metabolism Waxes / metabolism
IF 3.926
Times Cited 5
WOS Category HORTICULTURE AGRONOMY PLANT SCIENCES GENETICS & HEREDITY
Resource
Barley