RRC ID |
30205
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著者 |
Li C, Wang A, Ma X, Pourkheirandish M, Sakuma S, Wang N, Ning S, Nevo E, Nawrath C, Komatsuda T, Chen G.
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タイトル |
An eceriferum locus, cer-zv, is associated with a defect in cutin responsible for water retention in barley (Hordeum vulgare) leaves.
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ジャーナル |
Theor Appl Genet
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Abstract |
Drought limits plant growth and threatens crop productivity. A barley (Hordeum vulgare) ethylene imine-induced monogenic recessive mutant cer-zv, which is sensitive to drought, was characterized and genetically mapped in the present study. Detached leaves of cer-zv lost 34.2 % of their initial weight after 1 h of dehydration. The transpiration was much higher in cer-zv leaves than in wild-type leaves under both light and dark conditions. The stomata of cer-zv leaves functioned normally, but the cuticle of cer-zv leaves showed increased permeability to ethanol and toluidine blue dye. There was a 50-90 % reduction in four major cutin monomers, but no reduction in wax loads was found in the cer-zv mutant as compared with the wild type. Two F(2) mapping populations were established by the crosses of 23-19 × cer-zv and cer-zv × OUH602. More polymorphisms were found in EST sequences between cer-zv and OUH602 than between cer-zv and 23-19. cer-zv was located in a pericentromeric region on chromosome 4H in a 10.8 cM interval in the 23-19 × cer-zv map based on 186 gametes tested and a 1.7 cM interval in the cer-zv × OUH602 map based on 176 gametes tested. It co-segregated with EST marker AK251484 in both maps. The results indicated that the cer-zv mutant is defective in cutin, which might be responsible for the increased transpiration rate and drought sensitivity, and that the F(2) of cer-zv × OUH602 might better facilitate high resolution mapping of cer-zv.
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巻・号 |
126(3)
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ページ |
637-46
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公開日 |
2013-3-1
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DOI |
10.1007/s00122-012-2007-3
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PMID |
23124432
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MeSH |
Chlorophyll / metabolism
Chromosome Mapping
Chromosomes, Plant
DNA, Plant / genetics
Expressed Sequence Tags
Genes, Plant*
Genetic Linkage
Genetic Loci*
Genetic Markers
Genotype
Hordeum / genetics*
Hordeum / growth & development
Membrane Lipids / metabolism*
Mutation
Plant Leaves / genetics*
Plant Leaves / growth & development
Water / metabolism
Waxes / metabolism
|
IF |
4.439
|
引用数 |
10
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WOS 分野
|
HORTICULTURE
AGRONOMY
PLANT SCIENCES
GENETICS & HEREDITY
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リソース情報 |
オオムギ |
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