| RRC ID |
30206
|
| Author |
Koppolu R, Anwar N, Sakuma S, Tagiri A, Lundqvist U, Pourkheirandish M, Rutten T, Seiler C, Himmelbach A, Ariyadasa R, Youssef HM, Stein N, Sreenivasulu N, Komatsuda T, Schnurbusch T.
|
| Title |
Six-rowed spike4 (Vrs4) controls spikelet determinacy and row-type in barley.
|
| Journal |
Proc Natl Acad Sci U S A
|
| Abstract |
Inflorescence architecture of barley (Hordeum vulgare L.) is common among the Triticeae species, which bear one to three single-flowered spikelets at each rachis internode. Triple spikelet meristem is one of the unique features of barley spikes, in which three spikelets (one central and two lateral spikelets) are produced at each rachis internode. Fertility of the lateral spikelets at triple spikelet meristem gives row-type identity to barley spikes. Six-rowed spikes show fertile lateral spikelets and produce increased grain yield per spike, compared with two-rowed spikes with sterile lateral spikelets. Thus, far, two loci governing the row-type phenotype were isolated in barley that include Six-rowed spike1 (Vrs1) and Intermedium-C. In the present study, we isolated Six-rowed spike4 (Vrs4), a barley ortholog of the maize (Zea mays L.) inflorescence architecture gene RAMOSA2 (RA2). Eighteen coding mutations in barley RA2 (HvRA2) were specifically associated with lateral spikelet fertility and loss of spikelet determinacy. Expression analyses through mRNA in situ hybridization and microarray showed that Vrs4 (HvRA2) controls the row-type pathway through Vrs1 (HvHox1), a negative regulator of lateral spikelet fertility in barley. Moreover, Vrs4 may also regulate transcripts of barley SISTER OF RAMOSA3 (HvSRA), a putative trehalose-6-phosphate phosphatase involved in trehalose-6-phosphate homeostasis implicated to control spikelet determinacy. Our expression data illustrated that, although RA2 is conserved among different grass species, its down-stream target genes appear to be modified in barley and possibly other species of tribe Triticeae.
|
| Volume |
110(32)
|
| Pages |
13198-203
|
| Published |
2013-8-6
|
| DOI |
10.1073/pnas.1221950110
|
| PII |
1221950110
|
| PMID |
23878219
|
| PMC |
PMC3740847
|
| MeSH |
Amino Acid Sequence
Base Sequence
Chromosome Mapping
Chromosomes, Plant / genetics
Fertility / genetics
Gene Expression Profiling
Gene Expression Regulation, Plant*
Haplotypes
Hordeum / genetics*
Hordeum / metabolism
Hordeum / ultrastructure
Inflorescence / genetics*
Inflorescence / metabolism
Inflorescence / ultrastructure
Microscopy, Electron, Scanning
Molecular Sequence Data
Mutation
Oligonucleotide Array Sequence Analysis
Phenotype
Phylogeny
Plant Proteins / classification
Plant Proteins / genetics*
Plant Proteins / metabolism
Reverse Transcriptase Polymerase Chain Reaction
Sequence Homology, Amino Acid
|
| IF |
9.412
|
| Times Cited |
62
|
|
WOS Category
|
PLANT SCIENCES
|
| Resource |
| Barley |
|
