| RRC ID |
30207
|
| Author |
Minoura I, Hachikubo Y, Yamakita Y, Takazaki H, Ayukawa R, Uchimura S, Muto E.
|
| Title |
Overexpression, purification, and functional analysis of recombinant human tubulin dimer.
|
| Journal |
FEBS Lett
|
| Abstract |
Microtubules consisting of tubulin dimers play essential roles in various cellular functions. Investigating the structure-function relationship of tubulin dimers requires a method to prepare sufficient quantities of recombinant tubulin. To this end, we simultaneously expressed human α1- and β3-tubulin using a baculovirus-insect cell expression system that enabled the purification of 5mg recombinant tubulin per litre of cell culture. The purified recombinant human tubulin could be polymerized into microtubules that glide on a kinesin-coated glass surface. The method provides a powerful tool for in vitro functional analyses of microtubules.
|
| Volume |
587(21)
|
| Pages |
3450-5
|
| Published |
2013-11-1
|
| DOI |
10.1016/j.febslet.2013.08.032
|
| PII |
S0014-5793(13)00661-3
|
| PMID |
24021646
|
| MeSH |
Cells, Cultured
Humans
Kinesins / metabolism
Protein Multimerization
Recombinant Proteins / genetics*
Recombinant Proteins / isolation & purification*
Recombinant Proteins / metabolism
Tubulin / genetics*
Tubulin / isolation & purification*
Tubulin / metabolism
|
| IF |
3.057
|
| Times Cited |
50
|
|
WOS Category
|
BIOPHYSICS
BIOCHEMISTRY & MOLECULAR BIOLOGY
CELL BIOLOGY
|
| Resource |
| DNA material |
pFBD-Hs alpha1CGH/beta3CGF (RDB12753)
pFBD-Hs alpha1(K40R)CGH/beta3CGF (RDB12754). |
