RRC ID 30383
著者 Minoda A, Sakagami R, Yagisawa F, Kuroiwa T, Tanaka K.
タイトル Improvement of culture conditions and evidence for nuclear transformation by homologous recombination in a red alga, Cyanidioschyzon merolae 10D.
ジャーナル Plant Cell Physiol
Abstract Although the nuclear genome sequence of Cyanidioschyzon merolae 10D, a unicellular red alga, was recently determined, DNA transformation technology that is important as a model plant system has never been available thus far. In this study, improved culture conditions resulted in a faster growth rate of C. merolae in liquid medium (doubling time = 9.2 h), and colony formation on gellan gum plates. Using these conditions, spontaneous mutants (5-fluoroortic acid resistant) deficient in the UMP synthase gene were isolated. The lesions were then restored by introducing the wild-type UMP synthase gene into the cells suggesting DNA transformation by homologous recombination.
巻・号 45(6)
ページ 667-71
公開日 2004-6-1
DOI 10.1093/pcp/pch087
PMID 15215501
MeSH Amino Acid Sequence Base Sequence Cell Culture Techniques / methods* Cell Nucleus / genetics* Cells, Cultured Culture Media / pharmacology DNA, Plant / genetics* Molecular Sequence Data Multienzyme Complexes / deficiency Multienzyme Complexes / genetics Mutation / genetics Orotate Phosphoribosyltransferase / deficiency Orotate Phosphoribosyltransferase / genetics Orotic Acid / analogs & derivatives* Orotic Acid / pharmacology Orotidine-5'-Phosphate Decarboxylase / deficiency Orotidine-5'-Phosphate Decarboxylase / genetics Recombination, Genetic / genetics* Rhodophyta / genetics* Rhodophyta / metabolism* Transformation, Genetic / genetics*
IF 4.062
引用数 124
WOS 分野 PLANT SCIENCES CELL BIOLOGY
リソース情報
藻類 NIES-3377