論文 - 詳細
RRC ID | 30383 |
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著者 | Minoda A, Sakagami R, Yagisawa F, Kuroiwa T, Tanaka K. |
タイトル | Improvement of culture conditions and evidence for nuclear transformation by homologous recombination in a red alga, Cyanidioschyzon merolae 10D. |
ジャーナル | Plant Cell Physiol |
Abstract |
Although the nuclear genome sequence of Cyanidioschyzon merolae 10D, a unicellular red alga, was recently determined, DNA transformation technology that is important as a model plant system has never been available thus far. In this study, improved culture conditions resulted in a faster growth rate of C. merolae in liquid medium (doubling time = 9.2 h), and colony formation on gellan gum plates. Using these conditions, spontaneous mutants (5-fluoroortic acid resistant) deficient in the UMP synthase gene were isolated. The lesions were then restored by introducing the wild-type UMP synthase gene into the cells suggesting DNA transformation by homologous recombination. |
巻・号 | 45(6) |
ページ | 667-71 |
公開日 | 2004-6-1 |
DOI | 10.1093/pcp/pch087 |
PMID | 15215501 |
MeSH | Amino Acid Sequence Base Sequence Cell Culture Techniques / methods* Cell Nucleus / genetics* Cells, Cultured Culture Media / pharmacology DNA, Plant / genetics* Molecular Sequence Data Multienzyme Complexes / deficiency Multienzyme Complexes / genetics Mutation / genetics Orotate Phosphoribosyltransferase / deficiency Orotate Phosphoribosyltransferase / genetics Orotic Acid / analogs & derivatives* Orotic Acid / pharmacology Orotidine-5'-Phosphate Decarboxylase / deficiency Orotidine-5'-Phosphate Decarboxylase / genetics Recombination, Genetic / genetics* Rhodophyta / genetics* Rhodophyta / metabolism* Transformation, Genetic / genetics* |
IF | 4.062 |
引用数 | 124 |
WOS 分野 | PLANT SCIENCES CELL BIOLOGY |
リソース情報 | |
藻類 | NIES-3377 |