RRC ID 30417
著者 Tsuda L, Inoue YH, Yoo MA, Mizuno M, Hata M, Lim YM, Adachi-Yamada T, Ryo H, Masamune Y, Nishida Y.
タイトル A protein kinase similar to MAP kinase activator acts downstream of the raf kinase in Drosophila.
ジャーナル Cell
Abstract D-raf, a Drosophila homolog of Raf-1, plays key roles in multiple signal transduction pathways. Dsor1, a putative factor downstream of D-raf, was genetically identified by screening of dominant suppressors of D-raf. Dsor1Su1 mapped on X chromosome significantly suppressed the D-raf mutant phenotypes, and the loss-of-function mutations of Dsor1 showed phenotypes similar to those of the D-raf null mutations. Dsor1Su1 also significantly suppressed the mutations of other terminal class genes acting further upstream of D-raf. Molecular cloning of Dsor1 revealed its product with striking similarity to the microtubule-associated protein (MAP) kinase activator and yeast PBS2, STE7, and byr1. Our genetic results demonstrate the connection between raf and the highly conserved protein kinase cascade involving MAP kinase in vivo.
巻・号 72(3)
ページ 407-14
公開日 1993-2-12
DOI 10.1016/0092-8674(93)90117-9
PII 0092-8674(93)90117-9
PMID 8381718
MeSH Amino Acid Sequence Animals Calcium-Calmodulin-Dependent Protein Kinases Cell Division Cloning, Molecular Drosophila melanogaster / physiology* Gene Expression Genetic Complementation Test Molecular Sequence Data Phosphorylation Protein Kinases / genetics Protein Serine-Threonine Kinases / genetics* Protein Serine-Threonine Kinases / metabolism Proto-Oncogene Proteins / physiology* Proto-Oncogene Proteins c-raf Signal Transduction*
IF 38.637
引用数 202
WOS 分野 BIOCHEMISTRY & MOLECULAR BIOLOGY CELL BIOLOGY
リソース情報
ショウジョウバエ DGRC#109799