論文 - 詳細
| RRC ID | 30426 |
|---|---|
| 著者 | Adachi-Yamada T, Nakamura M, Irie K, Tomoyasu Y, Sano Y, Mori E, Goto S, Ueno N, Nishida Y, Matsumoto K. |
| タイトル | p38 mitogen-activated protein kinase can be involved in transforming growth factor beta superfamily signal transduction in Drosophila wing morphogenesis. |
| ジャーナル | Mol Cell Biol |
| Abstract |
p38 mitogen-activated protein kinase (p38) has been extensively studied as a stress-responsive kinase, but its role in development remains unknown. The fruit fly, Drosophila melanogaster, has two p38 genes, D-p38a and D-p38b. To elucidate the developmental function of the Drosophila p38's, we used various genetic and pharmacological manipulations to interfere with their functions: expression of a dominant-negative form of D-p38b, expression of antisense D-p38b RNA, reduction of the D-p38 gene dosage, and treatment with the p38 inhibitor SB203580. Expression of a dominant-negative D-p38b in the wing imaginal disc caused a decapentaplegic (dpp)-like phenotype and enhanced the phenotype of a dpp mutant. Dpp is a secretory ligand belonging to the transforming growth factor beta superfamily which triggers various morphogenetic processes through interaction with the receptor Thick veins (Tkv). Inhibition of D-p38b function also caused the suppression of the wing phenotype induced by constitutively active Tkv (TkvCA). Mosaic analysis revealed that D-p38b regulates the Tkv-dependent transcription of the optomotor-blind (omb) gene in non-Dpp-producing cells, indicating that the site of D-p38b action is downstream of Tkv. Furthermore, forced expression of TkvCA induced an increase in the phosphorylated active form(s) of D-p38(s). These results demonstrate that p38, in addition to its role as a transducer of emergency stress signaling, may function to modulate Dpp signaling. |
| 巻・号 | 19(3) |
| ページ | 2322-9 |
| 公開日 | 1999-3-1 |
| DOI | 10.1128/MCB.19.3.2322 |
| PMID | 10022918 |
| PMC | PMC84024 |
| MeSH | Animals Base Sequence Calcium-Calmodulin-Dependent Protein Kinases / genetics Calcium-Calmodulin-Dependent Protein Kinases / metabolism* DNA-Binding Proteins / genetics Drosophila Proteins* Drosophila melanogaster / genetics Drosophila melanogaster / growth & development Drosophila melanogaster / metabolism Insect Proteins / genetics Insect Proteins / metabolism* Mice Mice, Transgenic Mitogen-Activated Protein Kinases* Molecular Sequence Data Morphogenesis Nerve Tissue Proteins / genetics Phenotype Protein Serine-Threonine Kinases / genetics Protein Serine-Threonine Kinases / metabolism Receptors, Cell Surface / genetics Receptors, Cell Surface / metabolism Signal Transduction* T-Box Domain Proteins* Transcription, Genetic Transforming Growth Factor beta / genetics Transforming Growth Factor beta / metabolism* Wings, Animal / growth & development p38 Mitogen-Activated Protein Kinases |
| IF | 3.611 |
| 引用数 | 132 |
| WOS 分野 | BIOCHEMISTRY & MOLECULAR BIOLOGY CELL BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 各媒体での言及数の合計 | 0 |
| リソース情報 | |
| ショウジョウバエ | DGRC#109630 |