| RRC ID |
30429
|
| 著者 |
Baba K, Takeshita A, Majima K, Ueda R, Kondo S, Juni N, Yamamoto D.
|
| タイトル |
The Drosophila Bruton's tyrosine kinase (Btk) homolog is required for adult survival and male genital formation.
|
| ジャーナル |
Mol Cell Biol
|
| Abstract |
We isolated a Drosophila fickleP (ficP) mutant with a shortened copulatory duration and reduced adult-stage life span. The reduced copulatory duration is ascribable to incomplete fusion of the left and right halves of the apodeme that holds the penis during copulation. ficP is an intronic mutation occurring in the Btk gene, a gene which encodes two forms (type 1 and type 2) of a Bruton's tyrosine kinase (Btk) family cytoplasmic tyrosine kinase as a result of alternative exon usage. The ficP mutation prevents the formation of the type 2 isoform but leaves expression of the type 1 transcript intact. Ubiquitous overexpression of the wild-type cDNA by using a heat shock 70 promoter during the late larval or pupal stages rescued the life span and genital defects in the mutant, respectively, establishing the causal relationship between the ficP phenotypes and the Btk gene mutation. The stage specificity of the rescuing ability suggests that the Btk gene is required for the development of male genitalia and substrates required for adult survival.
|
| 巻・号 |
19(6)
|
| ページ |
4405-13
|
| 公開日 |
1999-6-1
|
| DOI |
10.1128/MCB.19.6.4405
|
| PMID |
10330180
|
| PMC |
PMC104399
|
| MeSH |
Agammaglobulinaemia Tyrosine Kinase
Age Factors
Alternative Splicing
Amino Acid Sequence
Animals
Drosophila melanogaster / genetics*
Genitalia, Male / anatomy & histology
Genitalia, Male / growth & development*
Longevity*
Male
Microscopy, Electron, Scanning
Models, Biological
Molecular Sequence Data
Mutation
Phenotype
Protein-Tyrosine Kinases / genetics*
Protein-Tyrosine Kinases / physiology*
Reverse Transcriptase Polymerase Chain Reaction
Sequence Homology, Amino Acid
Time Factors
Tissue Distribution
src-Family Kinases / genetics
|
| IF |
3.611
|
| 引用数 |
45
|
|
WOS 分野
|
BIOCHEMISTRY & MOLECULAR BIOLOGY
CELL BIOLOGY
|
| リソース情報 |
| ショウジョウバエ |
DGRC#109037 |
