論文 - 詳細
| RRC ID | 30429 |
|---|---|
| 著者 | Baba K, Takeshita A, Majima K, Ueda R, Kondo S, Juni N, Yamamoto D. |
| タイトル | The Drosophila Bruton's tyrosine kinase (Btk) homolog is required for adult survival and male genital formation. |
| ジャーナル | Mol Cell Biol |
| Abstract |
We isolated a Drosophila fickleP (ficP) mutant with a shortened copulatory duration and reduced adult-stage life span. The reduced copulatory duration is ascribable to incomplete fusion of the left and right halves of the apodeme that holds the penis during copulation. ficP is an intronic mutation occurring in the Btk gene, a gene which encodes two forms (type 1 and type 2) of a Bruton's tyrosine kinase (Btk) family cytoplasmic tyrosine kinase as a result of alternative exon usage. The ficP mutation prevents the formation of the type 2 isoform but leaves expression of the type 1 transcript intact. Ubiquitous overexpression of the wild-type cDNA by using a heat shock 70 promoter during the late larval or pupal stages rescued the life span and genital defects in the mutant, respectively, establishing the causal relationship between the ficP phenotypes and the Btk gene mutation. The stage specificity of the rescuing ability suggests that the Btk gene is required for the development of male genitalia and substrates required for adult survival. |
| 巻・号 | 19(6) |
| ページ | 4405-13 |
| 公開日 | 1999-6-1 |
| DOI | 10.1128/MCB.19.6.4405 |
| PMID | 10330180 |
| PMC | PMC104399 |
| MeSH | Agammaglobulinaemia Tyrosine Kinase Age Factors Alternative Splicing Amino Acid Sequence Animals Drosophila melanogaster / genetics* Genitalia, Male / anatomy & histology Genitalia, Male / growth & development* Longevity* Male Microscopy, Electron, Scanning Models, Biological Molecular Sequence Data Mutation Phenotype Protein-Tyrosine Kinases / genetics* Protein-Tyrosine Kinases / physiology* Reverse Transcriptase Polymerase Chain Reaction Sequence Homology, Amino Acid Time Factors Tissue Distribution src-Family Kinases / genetics |
| IF | 3.611 |
| 引用数 | 45 |
| WOS 分野 | BIOCHEMISTRY & MOLECULAR BIOLOGY CELL BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
|
| 最多言及媒体 | Wikipedia |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ショウジョウバエ | DGRC#109037 |