RRC ID |
3043
|
著者 |
Tanasupawat S, Pakdeeto A, Namwong S, Thawai C, Kudo T, Itoh T.
|
タイトル |
Lentibacillus halophilus sp. nov., from fish sauce in Thailand.
|
ジャーナル |
Int J Syst Evol Microbiol
|
Abstract |
Fifteen strains of extremely halophilic bacteria were isolated from fish sauce (nam-pla) collected in Thailand at various stages of the fish-fermentation process. The isolates were strictly aerobic, spore-forming, Gram-positive rods. They grew optimally in the presence of 20-26 % NaCl. The cell-wall peptidoglycan contained meso-diaminopimelic acid. The predominant menaquinone was MK-7. The major cellular fatty acids were anteiso-C(15 : 0) and anteiso-C(17 : 0). Polar lipid analysis revealed the presence of phosphatidylglycerol, diphosphatidylglycerol and two unidentified glycolipids. The DNA G+C content was 42.1-43.1 mol%. On the basis of the 16S rRNA gene sequence, a representative strain, PS11-2(T), was found to be closely related to Lentibacillus juripiscarius JCM 12147(T) (97.3 % similarity). The 15 strains were included in the same species on the basis that the levels of DNA-DNA relatedness with strain PS11-2(T) were greater than 70 %. They could be distinguished from L. juripiscarius and other Lentibacillus species on the basis of several phenotypic characteristics and low levels of DNA-DNA relatedness (=19.4 %). Therefore, the strains represent a novel species of the genus Lentibacillus, for which the name Lentibacillus halophilus sp. nov. is proposed. The type strain is PS11-2(T) (=JCM 12149(T)=TISTR 1549(T)=PCU 240(T)).
|
巻・号 |
56(Pt 8)
|
ページ |
1859-1863
|
公開日 |
2006-8-1
|
DOI |
10.1099/ijs.0.63997-0
|
PMID |
16902021
|
MeSH |
Animals
Bacillaceae / chemistry
Bacillaceae / classification*
Bacillaceae / genetics
Bacillaceae / growth & development
Bacillaceae / isolation & purification
Culture Media
Cyanoacrylates / analysis
DNA, Bacterial / genetics
Diaminopimelic Acid / analysis
Fatty Acids / analysis
Fermentation
Fish Products / microbiology*
Molecular Sequence Data
Nucleic Acid Hybridization
Phylogeny
RNA, Bacterial / genetics
RNA, Ribosomal, 16S / genetics
Sequence Homology, Nucleic Acid
Sodium Chloride
Species Specificity
Thailand
Vitamin K 2 / analogs & derivatives
|
IF |
2.415
|
引用数 |
31
|
WOS 分野
|
MICROBIOLOGY
|
リソース情報 |
病原微生物 |
JCM 12147?
JCM 12149? |