論文 - 詳細
| RRC ID | 30475 |
|---|---|
| 著者 | Li C, Vagin VV, Lee S, Xu J, Ma S, Xi H, Seitz H, Horwich MD, Syrzycka M, Honda BM, Kittler EL, Zapp ML, Klattenhoff C, Schulz N, Theurkauf WE, Weng Z, Zamore PD. |
| タイトル | Collapse of germline piRNAs in the absence of Argonaute3 reveals somatic piRNAs in flies. |
| ジャーナル | Cell |
| Abstract |
Piwi-interacting RNAs (piRNAs) silence transposons in animal germ cells. piRNAs are thought to derive from long transcripts spanning transposon-rich genomic loci and to direct an autoamplification loop in which an antisense piRNA, bound to Aubergine or Piwi protein, triggers production of a sense piRNA bound to the PIWI protein Argonaute3 (Ago3). In turn, the new piRNA is envisioned to produce a second antisense piRNA. Here, we describe strong loss-of-function mutations in ago3, allowing a direct genetic test of this model. We find that Ago3 acts to amplify piRNA pools and to enforce on them an antisense bias, increasing the number of piRNAs that can act to silence transposons. We also detect a second, Ago3-independent piRNA pathway centered on Piwi. Transposons targeted by this second pathway often reside in the flamenco locus, which is expressed in somatic ovarian follicle cells, suggesting a role for piRNAs beyond the germline. |
| 巻・号 | 137(3) |
| ページ | 509-21 |
| 公開日 | 2009-5-1 |
| DOI | 10.1016/j.cell.2009.04.027 |
| PII | S0092-8674(09)00452-8 |
| PMID | 19395009 |
| PMC | PMC2768572 |
| MeSH | Animals Argonaute Proteins Drosophila Proteins / genetics* Drosophila Proteins / metabolism* Drosophila melanogaster / genetics* Drosophila melanogaster / metabolism* Female Gene Silencing Mutation Ovarian Follicle / metabolism* Peptide Initiation Factors / genetics* Peptide Initiation Factors / metabolism* RNA, Small Interfering / metabolism Retroelements* |
| IF | 38.637 |
| 引用数 | 349 |
| WOS 分野 | BIOCHEMISTRY & MOLECULAR BIOLOGY CELL BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 8 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ショウジョウバエ | DGRC#109639 |