Reference - Detail
| RRC ID | 30476 |
|---|---|
| Author | Kaido M, Wada H, Shindo M, Hayashi S. |
| Title | Essential requirement for RING finger E3 ubiquitin ligase Hakai in early embryonic development of Drosophila. |
| Journal | Genes Cells |
| Abstract |
Hakai is a RING finger type E3 ubiquitin ligase that is highly conserved in metazoans. Mammalian Hakai was shown to bind and ubiquitinate the intracellular domain of E-cadherin, and this activity is implicated in down-regulation of E-cadherin during v-Src-induced cellular transformation. To evaluate this model in vivo, we studied the function of the Drosophila homologue of Hakai. In cultured S2 cells, Drosophila Hakai and E-cadherin (Shotgun) formed a complex in a way distinct from the interaction described for mammalian counterparts. Hakai null mutants died during larval stages but this lethality could be offset by a HA-tagged Hakai construct. While zygotic Hakai function was dispensable for cell proliferation and differentiation in the wing disc epithelium, maternal Hakai mutants showed a variety of defects in epithelial integrity, including stochastic loss of E-cadherin expression and reduction of aPKC; defects in cell specification and cell migration were also observed. No increase of E-cadherin, however, was observed. Regulation of multiple target proteins under control of Hakai is, therefore, essential for early embryonic morphogenesis in Drosophila. |
| Volume | 14(9) |
| Pages | 1067-77 |
| Published | 2009-9-1 |
| DOI | 10.1111/j.1365-2443.2009.01335.x |
| PII | GTC1335 |
| PMID | 19682089 |
| MeSH | Animals Cadherins / metabolism Cells, Cultured Drosophila / embryology* Drosophila / enzymology Drosophila / genetics Drosophila Proteins* / genetics Drosophila Proteins* / metabolism Embryo, Nonmammalian / metabolism Embryonic Development / genetics Gene Expression Regulation, Developmental* Mutation RING Finger Domains / genetics* Ubiquitin-Protein Ligases* / genetics Ubiquitin-Protein Ligases* / metabolism |
| IF | 1.655 |
| Times Cited | 21 |
| WOS Category | GENETICS & HEREDITY CELL BIOLOGY |
| Altmetric score |
オルトメトリクス指標項目
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| The most frequently cited source | X(Twitter) |
| Total number of mentions | 2 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| Drosophila | DGRC#109654 |