Reference - Detail
| RRC ID | 30484 |
|---|---|
| Author | Dong B, Kakihara K, Otani T, Wada H, Hayashi S. |
| Title | Rab9 and retromer regulate retrograde trafficking of luminal protein required for epithelial tube length control. |
| Journal | Nat Commun |
| Abstract |
Apical extracellular matrix filling the lumen controls the morphology and geometry of epithelial tubes during development, yet the regulation of luminal protein composition and its role in tube morphogenesis are not well understood. Here we show that an endosomal-retrieval machinery consisting of Rab9, retromer and actin nucleator WASH (Wiskott-Aldrich Syndrome Protein and SCAR Homolog) regulates selective recycling of the luminal protein Serpentine in the Drosophila trachea. Secreted Serpentine is endocytosed and sorted into the late endosome. Vps35, WASH and actin filaments differentially localize at the Rab9-enriched subdomains of the endosomal membrane, where Serpentine containing vesicles bud off. In Rab9, Vps35 and WASH mutants, Serpentine was secreted normally into the tracheal lumen, but the luminal quantities were depleted at later stages, resulting in excessively elongated tubes. In contrast, secretion of many luminal proteins was unaffected, suggesting that retrograde trafficking of a specific class of luminal proteins is a pivotal rate-limiting mechanism for continuous tube length regulation. |
| Volume | 4 |
| Pages | 1358 |
| Published | 2013-1-15 |
| DOI | 10.1038/ncomms2347 |
| PII | ncomms2347 |
| PMID | 23322046 |
| PMC | PMC3562448 |
| MeSH | Actins / metabolism Amidohydrolases / metabolism* Animals Drosophila Proteins / metabolism* Drosophila melanogaster / anatomy & histology* Drosophila melanogaster / cytology Drosophila melanogaster / embryology Drosophila melanogaster / metabolism Embryo, Nonmammalian / cytology Embryo, Nonmammalian / metabolism Endocytosis Endosomes / metabolism Epithelium / anatomy & histology* Epithelium / embryology Epithelium / metabolism Green Fluorescent Proteins / metabolism Multiprotein Complexes / metabolism* Protein Binding Protein Transport Trachea / anatomy & histology* Trachea / cytology Trachea / embryology Vesicular Transport Proteins / metabolism rab GTP-Binding Proteins / metabolism* trans-Golgi Network / metabolism |
| IF | 12.121 |
| Times Cited | 49 |
| WOS Category | CELL BIOLOGY |
| Resource | |
| Drosophila | DGRC#109833 |