| RRC ID |
30581
|
| 著者 |
Kimoto M, Tsubota T, Uchino K, Sezutsu H, Takiya S.
|
| タイトル |
Hox transcription factor Antp regulates sericin-1 gene expression in the terminal differentiated silk gland of Bombyx mori.
|
| ジャーナル |
Dev Biol
|
| Abstract |
Hox genes are well-known master regulators in developmental morphogenesis along the anteroposterior axis of animals. However, the molecular mechanisms by which Hox proteins regulate their target genes and determine cell fates are not fully understood. The silk gland of Bombyx mori is a tubular tissue divided into several subparts along the anteroposterior axis, and the silk genes are expressed with specific patterns. The sericin-1 gene (ser1) is expressed in the middle silk gland (MSG) with sublocal specificity. Here we show that the Hox protein Antp is a component of the middle silk gland-specific complex, MIC (MSG-intermolt-specific complex), binds to the essential promoter element of ser1, and activates its expression. Ectopic expression of Antp in transgenic silkworms induced the expression of ser1 in the posterior silk gland (PSG), but not in the anterior part of MSG (MSG-A). Correspondingly, a MIC-like complex was formed by the addition of recombinant Antp in extracts from PSG with its cofactors Exd and Hth, but not in extracts from MSG-A. Splicing patterns of ser1 mRNA induced by the ectopic expression of Antp in PSG were almost the same as those in MSG at the fifth instar and altered depending on the induction timing of Antp. Other Hox genes were expressed with sublocal specificity in the silk gland. The Bombyx silk gland might provide a useful system for understanding how Hox proteins select and regulate their target genes.
|
| 巻・号 |
386(1)
|
| ページ |
64-71
|
| 公開日 |
2014-2-1
|
| DOI |
10.1016/j.ydbio.2013.12.002
|
| PII |
S0012-1606(13)00647-7
|
| PMID |
24333180
|
| MeSH |
Animals
Animals, Genetically Modified
Base Sequence
Bombyx / genetics*
Bombyx / metabolism
Cell Differentiation
Cloning, Molecular
Female
Gene Expression Profiling
Gene Expression Regulation, Developmental*
Homeodomain Proteins / metabolism*
In Situ Hybridization
Larva
Male
Molecular Sequence Data
Promoter Regions, Genetic
RNA Splicing
Recombinant Proteins / metabolism
Sericins / metabolism*
Silk / metabolism
Tissue Distribution
Transgenes
|
| IF |
2.896
|
| 引用数 |
17
|
|
WOS 分野
|
DEVELOPMENTAL BIOLOGY
|
| リソース情報 |
| カイコ |
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