| RRC ID |
30597
|
| 著者 |
Nakamura S, Watakabe I, Nishimura T, Picard JY, Toyoda A, Taniguchi Y, di Clemente N, Tanaka M.
|
| タイトル |
Hyperproliferation of mitotically active germ cells due to defective anti-Müllerian hormone signaling mediates sex reversal in medaka.
|
| ジャーナル |
Development
|
| Abstract |
The function of AMH (Anti-Müllerian hormone), a phylogenetically ancient member of the TGFβ family of proteins, in lower vertebrates is largely unknown. Previously, we have shown that the gene encoding the type II anti-Müllerian hormone receptor, amhrII, is responsible for excessive germ cell proliferation and male-to-female sex reversal in the medaka hotei mutant. In this study, functional analyses in cultured cells and of other amhrII mutant alleles indicate that lack of AMH signaling causes the hotei phenotype. BrdU incorporation experiments identified the existence of both quiescent and mitotically active germ cells among the self-renewing, type I population of germ cells in the developing gonad. AMH signaling acts in supporting cells to promote the proliferation of mitotically active germ cells but does not trigger quiescent germ cells to proliferate in the developing gonad. Furthermore, we show that the male-to-female sex reversal phenotype in hotei mutants is not a direct consequence of AMH signaling in supporting cells, but is instead mediated by germ cells. Our data demonstrate that interfollicular AMH signaling regulates proliferation at a specific stage of germ cell development, and that this regulation is crucial for the proper manifestation of gonadal sex directed by sex determination genes.
|
| 巻・号 |
139(13)
|
| ページ |
2283-7
|
| 公開日 |
2012-7-1
|
| DOI |
10.1242/dev.076307
|
| PII |
dev.076307
|
| PMID |
22627284
|
| MeSH |
Animals
Anti-Mullerian Hormone / physiology*
Cell Proliferation*
Cells, Cultured
Female
Germ Cells / cytology*
Germ Cells / physiology
Male
Mitosis
Mutation
Oryzias / growth & development*
Oryzias / metabolism
Receptors, Peptide / genetics
Receptors, Peptide / metabolism*
Receptors, Transforming Growth Factor beta / genetics
Receptors, Transforming Growth Factor beta / metabolism*
Sex Differentiation*
Signal Transduction
|
| IF |
5.611
|
| 引用数 |
49
|
|
WOS 分野
|
DEVELOPMENTAL BIOLOGY
|
| リソース情報 |
| メダカ |
Hatching enzyme
MT436
TILLING_MUTANT |
