論文 - 詳細
| RRC ID | 30606 |
|---|---|
| 著者 | Ohshima A, Morimura N, Matsumoto C, Hiraga A, Komine R, Kimura T, Naruse K, Fukamachi S. |
| タイトル | Effects of body-color mutations on vitality: an attempt to establish easy-to-breed see-through medaka strains by outcrossing. |
| ジャーナル | G3 (Bethesda) |
| Abstract |
"See-through" strains of medaka are unique tools for experiments: their skin is transparent, and their internal organs can be externally monitored throughout life. However, see-through fish are less vital than normally pigmented wild-type fish, which allows only skilled researchers to make the most of their advantages. Expecting that hybrid vigor (heterosis) would increase the vitality, we outcrossed two see-through strains (SK(2) and STIII) with a genetically distant wild-type strain (HNI). Fish with the see-through phenotypes were successfully restored in the F2 generation and maintained as closed colonies. We verified that genomes of these hybrid see-through strains actually consisted of approximately 50% HNI and approximately 50% SK(2) or STIII alleles, but we could not obtain evidence supporting improved survival of larvae or fecundity of adults, at least under our breeding conditions. We also found that four of the five see-through mutations (b(g8), i-3, gu, and il-1 but not lf) additively decrease viability. Given that heterosis could not overwhelm the viability-reducing effects of the see-through mutations, easy-to-breed see-through strains will only be established by other methods such as conditional gene targeting or screening of new body-color mutations that do not reduce viability. |
| 巻・号 | 3(9) |
| ページ | 1577-85 |
| 公開日 | 2013-9-4 |
| DOI | 10.1534/g3.113.007575 |
| PII | g3.113.007575 |
| PMID | 23893740 |
| PMC | PMC3755918 |
| MeSH | Alleles Animals Breeding Crossing Over, Genetic* Genome Genotype Hybrid Vigor Longevity Membrane Transport Proteins / genetics Mutation Oryzias / genetics* Phenotype Skin Pigmentation / genetics* |
| IF | 2.781 |
| 引用数 | 3 |
| WOS 分野 | GENETICS & HEREDITY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| メダカ | STIII (MT117) suke-suke (sk2) (MT959) |