RRC ID 30609
Author Ng GH, Gong Z.
Title GFP transgenic medaka (Oryzias latipes) under the inducible cyp1a promoter provide a sensitive and convenient biological indicator for the presence of TCDD and other persistent organic chemicals.
Journal PLoS ONE
Abstract Persistent organic pollutants (POPs) are resistant to environmental degradation and can cause multitude of health problems. Cytochrome P450 1A (Cyp1a) is often up-regulated by POPs through the activation of aryl hydrocarbon receptor (AhR) pathway and is thus usually used as a biomarker for xenobiotics exposure. To develop a convenient in vivo tool to monitor xenobiotic contamination in the water, we have established GFP transgenic medaka using the inducible cyp1a promoter, Tg(cyp1a:gfp). Here we tested Tg(cyp1a:gfp) medaka at three different stages, prehatching embryos, newly hatched fry and adult with 2,3,7,8-tetrachlorodiebnzo-p-dioxin (TCDD), a dioxin. While GFP induction was observed in all three stages, newly hatched fry were the most sensitive with the lowest observed effective concentration of 0.005 nM or 16.1 ng/L. The highly sensitive organs included the kidney, liver and intestine. With high concentrations of TCDD, several other organs such as the olfactory pit, tail fin, gills, lateral line neuromast cells and blood vessels also showed GFP expression. In addition, Tg(cyp1a:gfp) medaka fry also responded to two other AhR agonists, 3-methylcholanthrene and benzo[a]pyrene, for GFP induction, but no significant GFP induction was observed towards several other chemicals tested, indicating the specificity of this transgenic line. The GFP inducibility of Tg(cyp1a:gfp) medaka at both fry and adult stages may be useful for development of high-throughput assays as well as online water monitoring system to detect xenobiotic toxicity.
Volume 8(5)
Pages e64334
Published 2013
DOI 10.1371/journal.pone.0064334
PII PONE-D-13-06494
PMID 23700472
PMC PMC3659123
MeSH Animals Animals, Genetically Modified Aryl Hydrocarbon Hydroxylases / genetics* Embryo, Nonmammalian / drug effects Embryo, Nonmammalian / metabolism Green Fluorescent Proteins / biosynthesis* Green Fluorescent Proteins / genetics Intestines / drug effects Intestines / metabolism Kidney / drug effects Kidney / metabolism Liver / drug effects Liver / metabolism Organ Specificity Oryzias / genetics* Oryzias / metabolism Polychlorinated Dibenzodioxins / analysis* Polychlorinated Dibenzodioxins / pharmacology Promoter Regions, Genetic Transcriptional Activation / drug effects Up-Regulation Water Pollutants, Chemical / analysis* Water Pollutants, Chemical / pharmacology
IF 2.776
Times Cited 10
Medaka Hd-rRII1(IB178)