論文 - 詳細
| RRC ID | 30628 |
|---|---|
| 著者 | Bagijn MP, Goldstein LD, Sapetschnig A, Weick EM, Bouasker S, Lehrbach NJ, Simard MJ, Miska EA. |
| タイトル | Function, targets, and evolution of Caenorhabditis elegans piRNAs. |
| ジャーナル | Science |
| Abstract |
Piwi-interacting RNAs (piRNAs) are small RNAs required to maintain germline integrity and fertility, but their mechanism of action is poorly understood. Here we demonstrate that Caenorhabditis elegans piRNAs silence transcripts in trans through imperfectly complementary sites. Target silencing is independent of Piwi endonuclease activity or "slicing." Instead, piRNAs initiate a localized secondary endogenous small interfering RNA (endo-siRNA) response. Endogenous protein-coding gene and transposon transcripts exhibit Piwi-dependent endo-siRNAs at sites complementary to piRNAs and are derepressed in Piwi mutants. Genomic loci of piRNA biogenesis are depleted of protein-coding genes and tend to overlap the start and end of transposons in sense and antisense, respectively. Our data suggest that nematode piRNA clusters are evolving to generate piRNAs against active mobile elements. Thus, piRNAs provide heritable, sequence-specific triggers for RNA interference in C. elegans. |
| 巻・号 | 337(6094) |
| ページ | 574-578 |
| 公開日 | 2012-8-3 |
| DOI | 10.1126/science.1220952 |
| PMID | 22700655 |
| PMC | PMC3951736 |
| MeSH | Animals Argonaute Proteins / genetics Caenorhabditis elegans / genetics* Caenorhabditis elegans Proteins / genetics Evolution, Molecular Mutation RNA Interference* RNA, Double-Stranded / biosynthesis RNA, Double-Stranded / genetics RNA, Small Interfering / genetics RNA, Small Interfering / metabolism* Transcription, Genetic* |
| IF | 41.846 |
| 引用数 | 183 |
| WOS 分野 | BIOCHEMISTRY & MOLECULAR BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 44 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 線虫 | tm1116 |