RRC ID 30631
Author Cruciat CM, Dolde C, de Groot RE, Ohkawara B, Reinhard C, Korswagen HC, Niehrs C.
Title RNA helicase DDX3 is a regulatory subunit of casein kinase 1 in Wnt-β-catenin signaling.
Journal Science
Abstract Casein kinase 1 (CK1) members play key roles in numerous biological processes. They are considered "rogue" kinases, because their enzymatic activity appears unregulated. Contrary to this notion, we have identified the DEAD-box RNA helicase DDX3 as a regulator of the Wnt-β-catenin network, where it acts as a regulatory subunit of CK1ε: In a Wnt-dependent manner, DDX3 binds CK1ε and directly stimulates its kinase activity, and promotes phosphorylation of the scaffold protein dishevelled. DDX3 is required for Wnt-β-catenin signaling in mammalian cells and during Xenopus and Caenorhabditis elegans development. The results also suggest that the kinase-stimulatory function extends to other DDX and CK1 members, opening fresh perspectives for one of the longest-studied protein kinase families.
Volume 339(6126)
Pages 1436-41
Published 2013-3-22
DOI 10.1126/science.1231499
PII science.1231499
PMID 23413191
MeSH Adaptor Proteins, Signal Transducing / metabolism Animals Caenorhabditis elegans / genetics Caenorhabditis elegans / growth & development Caenorhabditis elegans / metabolism Caenorhabditis elegans Proteins / genetics Caenorhabditis elegans Proteins / metabolism Casein Kinase Iepsilon / chemistry Casein Kinase Iepsilon / metabolism* DEAD-box RNA Helicases / chemistry DEAD-box RNA Helicases / genetics DEAD-box RNA Helicases / metabolism* Dishevelled Proteins HEK293 Cells Humans Phosphoproteins / metabolism Phosphorylation Protein Binding Protein Structure, Tertiary RNA Helicases / chemistry RNA Helicases / genetics RNA Helicases / metabolism* Wnt Proteins / metabolism Wnt Signaling Pathway* Xenopus / embryology Xenopus / genetics Xenopus / metabolism Xenopus Proteins / chemistry Xenopus Proteins / genetics Xenopus Proteins / metabolism* beta Catenin / metabolism
IF 41.063
Times Cited 65
C.elegans tm1320