| RRC ID |
30677
|
| 著者 |
De Gregorio E, Han SJ, Lee WJ, Baek MJ, Osaki T, Kawabata S, Lee BL, Iwanaga S, Lemaitre B, Brey PT.
|
| タイトル |
An immune-responsive Serpin regulates the melanization cascade in Drosophila.
|
| ジャーナル |
Dev Cell
|
| Abstract |
In arthropods, the melanization reaction is associated with multiple host defense mechanisms leading to the sequestration and killing of invading microorganisms. Arthropod melanization is controlled by a cascade of serine proteases that ultimately activates the enzyme prophenoloxidase (PPO), which, in turn, catalyzes the synthesis of melanin. Here we report the biochemical and genetic characterization of a Drosophila serine protease inhibitor protein, Serpin-27A, which regulates the melanization cascade through the specific inhibition of the terminal protease prophenoloxidase-activating enzyme. Our data demonstrate that Serpin-27A is required to restrict the phenoloxidase activity to the site of injury or infection, preventing the insect from excessive melanization.
|
| 巻・号 |
3(4)
|
| ページ |
581-92
|
| 公開日 |
2002-10-1
|
| DOI |
10.1016/s1534-5807(02)00267-8
|
| PII |
S1534-5807(02)00267-8
|
| PMID |
12408809
|
| MeSH |
Amino Acid Sequence
Animals
Catechol Oxidase / immunology
Catechol Oxidase / metabolism
Drosophila Proteins / genetics
Drosophila Proteins / immunology*
Drosophila melanogaster / genetics
Drosophila melanogaster / immunology*
Enzyme Precursors / immunology
Enzyme Precursors / metabolism
Melanins / immunology
Melanins / metabolism
Molecular Sequence Data
Mutation
Serpins / genetics
Serpins / immunology*
Signal Transduction / immunology
|
| IF |
10.092
|
| 引用数 |
249
|
|
WOS 分野
|
DEVELOPMENTAL BIOLOGY
CELL BIOLOGY
|
| リソース情報 |
| ショウジョウバエ |
|
