Reference - Detail
| RRC ID | 30787 |
|---|---|
| Author | Nakagawa M, Kagiyama M, Shibata N, Hirano Y, Hakoshima T. |
| Title | Mechanism of high-affinity abscisic acid binding to PYL9/RCAR1. |
| Journal | Genes Cells |
| Abstract |
Arabidopsis receptors of abscisic acid (ABA), the key plant hormone for adaptation to water stress, comprise 14 PYR/PYLs/RCARs proteins classified into three subfamilies I, II, and III, which suggests functional differentiation. Although their monomer-dimer equilibria may be correlated with differences in their ABA-binding affinities, how the dimerization decreases the affinity is unclear. Comparative structural and binding studies between PYL9, which is a representative of high-affinity subfamily I, and low-affinity members of subfamily III reveals that the nonpolar triplet (Ile110, Val162, and Leu165) and Pro64 contribute to enhance ABA-binding affinity by inducing a shift of the ABA carboxyl group to form additional direct hydrogen bonds with conserved Asn169. Our mutation studies of PYL1 successfully produced a monomeric mutant PYL1 exhibiting low ABA affinity and also a dimeric mutant PYL1 exhibiting high ABA-binding affinity, suggesting that dimer formation of ABA receptors is not essential for their low ABA-binding affinity. Our study contributes toward establishing the structural basis for the higher ABA-binding affinity of the subfamily receptors and provides a clue for understanding the broad spectrum of hormone actions in plants manifested by the different hormone-binding affinity of multiple receptors. |
| Volume | 19(5) |
| Pages | 386-404 |
| Published | 2014-5-1 |
| DOI | 10.1111/gtc.12140 |
| PMID | 24645846 |
| MeSH | Abscisic Acid / metabolism* Amino Acid Sequence Arabidopsis / metabolism* Arabidopsis Proteins / genetics Arabidopsis Proteins / metabolism* Carrier Proteins / genetics Carrier Proteins / metabolism* Intracellular Signaling Peptides and Proteins Molecular Sequence Data Mutation Protein Binding Protein Multimerization |
| IF | 1.655 |
| Times Cited | 14 |
| WOS Category | GENETICS & HEREDITY CELL BIOLOGY |
| Altmetric score |
オルトメトリクス指標項目
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| The most frequently cited source | Patent(IFI CLAIMS) |
| Total number of mentions | 3 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| Arabidopsis / Cultured plant cells, genes | pda00088 pda01246 pda03718 pda03766 pda12991 pda18331 |