論文 - 詳細
| RRC ID | 30787 |
|---|---|
| 著者 | Nakagawa M, Kagiyama M, Shibata N, Hirano Y, Hakoshima T. |
| タイトル | Mechanism of high-affinity abscisic acid binding to PYL9/RCAR1. |
| ジャーナル | Genes Cells |
| Abstract |
Arabidopsis receptors of abscisic acid (ABA), the key plant hormone for adaptation to water stress, comprise 14 PYR/PYLs/RCARs proteins classified into three subfamilies I, II, and III, which suggests functional differentiation. Although their monomer-dimer equilibria may be correlated with differences in their ABA-binding affinities, how the dimerization decreases the affinity is unclear. Comparative structural and binding studies between PYL9, which is a representative of high-affinity subfamily I, and low-affinity members of subfamily III reveals that the nonpolar triplet (Ile110, Val162, and Leu165) and Pro64 contribute to enhance ABA-binding affinity by inducing a shift of the ABA carboxyl group to form additional direct hydrogen bonds with conserved Asn169. Our mutation studies of PYL1 successfully produced a monomeric mutant PYL1 exhibiting low ABA affinity and also a dimeric mutant PYL1 exhibiting high ABA-binding affinity, suggesting that dimer formation of ABA receptors is not essential for their low ABA-binding affinity. Our study contributes toward establishing the structural basis for the higher ABA-binding affinity of the subfamily receptors and provides a clue for understanding the broad spectrum of hormone actions in plants manifested by the different hormone-binding affinity of multiple receptors. |
| 巻・号 | 19(5) |
| ページ | 386-404 |
| 公開日 | 2014-5-1 |
| DOI | 10.1111/gtc.12140 |
| PMID | 24645846 |
| MeSH | Abscisic Acid / metabolism* Amino Acid Sequence Arabidopsis / metabolism* Arabidopsis Proteins / genetics Arabidopsis Proteins / metabolism* Carrier Proteins / genetics Carrier Proteins / metabolism* Intracellular Signaling Peptides and Proteins Molecular Sequence Data Mutation Protein Binding Protein Multimerization |
| IF | 1.655 |
| 引用数 | 14 |
| WOS 分野 | GENETICS & HEREDITY CELL BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 3 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| シロイヌナズナ / 植物培養細胞・遺伝子 | pda00088 pda01246 pda03718 pda03766 pda12991 pda18331 |