論文 - 詳細
| RRC ID | 30797 |
|---|---|
| 著者 | Wälde S, King MC. |
| タイトル | The KASH protein Kms2 coordinates mitotic remodeling of the spindle pole body. |
| ジャーナル | J Cell Sci |
| Abstract |
Defects in the biogenesis of the spindle pole body (SPB), the yeast centrosome equivalent, can lead to monopolar spindles and mitotic catastrophe. The KASH domain protein Kms2 and the SUN domain protein Sad1 colocalize within the nuclear envelope at the site of SPB attachment during interphase and at the spindle poles during mitosis in Schizosaccharomyces pombe. We show that Kms2 interacts with the essential SPB components Cut12 and Pcp1 and the Polo kinase Plo1. Depletion of Kms2 delays mitotic entry and leads to defects in the insertion of the SPB into the nuclear envelope, disrupting stable bipolar spindle formation. These effects are mediated in part by a delay in the recruitment of Plo1 to the SPB at mitotic entry. Plo1 activity supports mitotic SPB remodeling by driving a burst of incorporation of Cut12 and Pcp1. Thus, a fission yeast SUN-KASH complex plays an important role in supporting the remodeling of the SPB at mitotic entry. |
| 巻・号 | 127(Pt 16) |
| ページ | 3625-40 |
| 公開日 | 2014-8-15 |
| DOI | 10.1242/jcs.154997 |
| PII | jcs.154997 |
| PMID | 24963130 |
| PMC | PMC4132395 |
| MeSH | Cell Cycle Proteins / genetics Cell Cycle Proteins / metabolism* Microtubule-Associated Proteins / genetics Microtubule-Associated Proteins / metabolism Mitosis* Nuclear Proteins / genetics Nuclear Proteins / metabolism Phosphoproteins / genetics Phosphoproteins / metabolism Protein Binding Protein Serine-Threonine Kinases / genetics Protein Serine-Threonine Kinases / metabolism Schizosaccharomyces / cytology* Schizosaccharomyces / genetics Schizosaccharomyces / metabolism* Schizosaccharomyces pombe Proteins / genetics Schizosaccharomyces pombe Proteins / metabolism* Spindle Pole Bodies / genetics Spindle Pole Bodies / metabolism* |
| IF | 4.573 |
| 引用数 | 19 |
| WOS 分野 | CELL BIOLOGY |
| リソース情報 | |
| 酵母 | FY17669 |