RRC ID 30830
Author Lei B, Zhou N, Guo Y, Zhao W, Tan YW, Yu Y, Lu H.
Title Septin ring assembly is regulated by Spt20, a structural subunit of the SAGA complex.
Journal J Cell Sci
Abstract Accurate cell division requires the proper assembly of high-order septin structures. In fission yeast (Schizosaccharomyces pombe), Spn1-Spn4 are assembled into a primary septin ring at the division site, and the subsequent recruitment of Mid2 to the structure results in a stable septin ring. However, not much is known about the regulation of this key process. Here, we found that deletion of Spt20, a structural subunit of the Spt-Ada-Gcn5-acetyltransferase (SAGA) transcriptional activation complex, caused a severe cell separation defect. The defect was mainly due to impaired septin ring assembly, as 80% of spt20Δ cells lost septin rings at the division sites. Spt20 regulates septin ring assembly partially through the transcriptional activation of mid2(+). Spt20 also interacted with Spn2 and Mid2 in vitro and was associated with other components of the ring in vivo. Spt20 colocalized with the septin ring, but did not separate when the septin ring split. Importantly, Spt20 regulated the stability of the septin ring and was required for the recruitment of Mid2. The transcription-dependent and -independent roles of Spt20 in septin ring assembly highlight a multifaceted regulation of one process by a SAGA subunit.
Volume 127(Pt 18)
Pages 4024-36
Published 2014-9-15
DOI 10.1242/jcs.151910
PII jcs.151910
PMID 25015293
MeSH Cell Division Intracellular Signaling Peptides and Proteins / genetics Intracellular Signaling Peptides and Proteins / metabolism Membrane Glycoproteins / genetics Membrane Glycoproteins / metabolism Protein Transport Saccharomyces cerevisiae / cytology Saccharomyces cerevisiae / enzymology Saccharomyces cerevisiae / genetics Saccharomyces cerevisiae / metabolism* Saccharomyces cerevisiae Proteins / genetics Saccharomyces cerevisiae Proteins / metabolism* Septins / chemistry Septins / genetics Septins / metabolism* Trans-Activators / genetics Trans-Activators / metabolism* Transcription Factors / genetics Transcription Factors / metabolism*
IF 4.573
Times Cited 2
Yeast FY14936 FY14998 FY7266