論文 - 詳細
| RRC ID | 30830 |
|---|---|
| 著者 | Lei B, Zhou N, Guo Y, Zhao W, Tan YW, Yu Y, Lu H. |
| タイトル | Septin ring assembly is regulated by Spt20, a structural subunit of the SAGA complex. |
| ジャーナル | J Cell Sci |
| Abstract |
Accurate cell division requires the proper assembly of high-order septin structures. In fission yeast (Schizosaccharomyces pombe), Spn1-Spn4 are assembled into a primary septin ring at the division site, and the subsequent recruitment of Mid2 to the structure results in a stable septin ring. However, not much is known about the regulation of this key process. Here, we found that deletion of Spt20, a structural subunit of the Spt-Ada-Gcn5-acetyltransferase (SAGA) transcriptional activation complex, caused a severe cell separation defect. The defect was mainly due to impaired septin ring assembly, as 80% of spt20Δ cells lost septin rings at the division sites. Spt20 regulates septin ring assembly partially through the transcriptional activation of mid2(+). Spt20 also interacted with Spn2 and Mid2 in vitro and was associated with other components of the ring in vivo. Spt20 colocalized with the septin ring, but did not separate when the septin ring split. Importantly, Spt20 regulated the stability of the septin ring and was required for the recruitment of Mid2. The transcription-dependent and -independent roles of Spt20 in septin ring assembly highlight a multifaceted regulation of one process by a SAGA subunit. |
| 巻・号 | 127(Pt 18) |
| ページ | 4024-36 |
| 公開日 | 2014-9-15 |
| DOI | 10.1242/jcs.151910 |
| PII | jcs.151910 |
| PMID | 25015293 |
| MeSH | Cell Division Intracellular Signaling Peptides and Proteins / genetics Intracellular Signaling Peptides and Proteins / metabolism Membrane Glycoproteins / genetics Membrane Glycoproteins / metabolism Protein Transport Saccharomyces cerevisiae / cytology Saccharomyces cerevisiae / enzymology Saccharomyces cerevisiae / genetics Saccharomyces cerevisiae / metabolism* Saccharomyces cerevisiae Proteins / genetics Saccharomyces cerevisiae Proteins / metabolism* Septins / chemistry Septins / genetics Septins / metabolism* Trans-Activators / genetics Trans-Activators / metabolism* Transcription Factors / genetics Transcription Factors / metabolism* |
| IF | 4.573 |
| 引用数 | 2 |
| WOS 分野 | CELL BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 2 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 酵母 | FY14936 FY14998 FY7266 |