RRC ID 30843
著者 Moriyama A, Inohaya K, Maruyama K, Kudo A.
タイトル Bef medaka mutant reveals the essential role of c-myb in both primitive and definitive hematopoiesis.
ジャーナル Dev Biol
Abstract Vertebrate hematopoiesis is characterized by two evolutionally conserved phases of development, i.e., primitive hematopoiesis, which is a transient phenomenon in the early embryo, and definitive hematopoiesis, which takes place in the later stages. Beni fuji (bef) was originally isolated as a medaka mutant that has an apparently reduced number of erythrocytes in its peripheral blood. Positional cloning revealed that the bef mutant has a nonsense mutation in the c-myb gene. Previous studies have shown that c-myb is essential for definitive hematopoiesis, and c-myb is now widely used as a marker gene for the onset of definitive hematopoiesis. To analyze the phenotypes of the bef mutant, we performed whole-mount in situ hybridization with gene markers of hematopoietic cells. The bef embryos showed decreased expression of alpha-globin and l-plastin, and a complete loss of mpo1 and rag1 expression, suggesting that the bef embryos had defects not only in erythrocytes but also in other myeloid cells, which indicates that their definitive hematopoiesis was aberrant. Interestingly, we observed a diminution in the number of primitive erythrocytes and a delay in the emergence of primitive macrophages in the bef embryos. These results suggest that c-myb also functions in the primitive hematopoiesis, potentially demonstrating a link between primitive and definitive hematopoiesis.
巻・号 345(2)
ページ 133-43
公開日 2010-9-15
DOI 10.1016/j.ydbio.2010.06.031
PII S0012-1606(10)00904-8
PMID 20621080
MeSH Animals Embryo, Nonmammalian / metabolism Fish Proteins / genetics* Gene Expression Regulation, Developmental Hematopoiesis / genetics* In Situ Hybridization Macrophages / metabolism Membrane Glycoproteins / genetics Microfilament Proteins / genetics Mutation* Oryzias / genetics* Proto-Oncogene Proteins c-myb / genetics*
IF 2.896
引用数 14
WOS 分野 DEVELOPMENTAL BIOLOGY
リソース情報
メダカ ki14, beni fuji (MT329)