| RRC ID |
30900
|
| Author |
Velu TJ, Beguinot L, Vass WC, Willingham MC, Merlino GT, Pastan I, Lowy DR.
|
| Title |
Epidermal-growth-factor-dependent transformation by a human EGF receptor proto-oncogene.
|
| Journal |
Science
|
| Abstract |
The epidermal growth factor (EGF) receptor gene EGFR has been placed in a retrovirus vector to examine the growth properties of cells that experimentally overproduce a full-length EGF receptor. NIH 3T3 cells transfected with the viral DNA or infected with the corresponding rescued retrovirus developed a fully transformed phenotype in vitro that required both functional EGFR expression and the presence of EGF in the growth medium. Cells expressing 4 x 10(5) EGF receptors formed tumors in nude mice, while control cells did not. Therefore, the EGFR retrovirus, which had a titer on NIH 3T3 cells that was greater than 10(7) focus-forming units per milliliter, can efficiently transfer and express this gene, and increased numbers of EGF receptors can contribute to the transformed phenotype.
|
| Volume |
238(4832)
|
| Pages |
1408-10
|
| Published |
1987-12-4
|
| DOI |
10.1126/science.3500513
|
| PMID |
3500513
|
| MeSH |
Animals
Cell Transformation, Neoplastic / chemically induced
Cell Transformation, Neoplastic / genetics*
Cells, Cultured
DNA, Recombinant
Epidermal Growth Factor / pharmacology*
ErbB Receptors / drug effects
ErbB Receptors / genetics*
Fibroblasts / pathology
Genetic Vectors
Harvey murine sarcoma virus / genetics
Humans
Male
Mice
Mice, Nude
Neoplasms, Experimental / etiology
Proto-Oncogene Mas
Proto-Oncogenes*
Recombinant Proteins / genetics
|
| IF |
41.846
|
| Times Cited |
436
|
|
WOS Category
|
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
| Resource |
| DNA material |
pco12 EGFR (RDB01276) |
