Reference - Detail
| RRC ID | 30900 |
|---|---|
| Author | Velu TJ, Beguinot L, Vass WC, Willingham MC, Merlino GT, Pastan I, Lowy DR. |
| Title | Epidermal-growth-factor-dependent transformation by a human EGF receptor proto-oncogene. |
| Journal | Science |
| Abstract |
The epidermal growth factor (EGF) receptor gene EGFR has been placed in a retrovirus vector to examine the growth properties of cells that experimentally overproduce a full-length EGF receptor. NIH 3T3 cells transfected with the viral DNA or infected with the corresponding rescued retrovirus developed a fully transformed phenotype in vitro that required both functional EGFR expression and the presence of EGF in the growth medium. Cells expressing 4 x 10(5) EGF receptors formed tumors in nude mice, while control cells did not. Therefore, the EGFR retrovirus, which had a titer on NIH 3T3 cells that was greater than 10(7) focus-forming units per milliliter, can efficiently transfer and express this gene, and increased numbers of EGF receptors can contribute to the transformed phenotype. |
| Volume | 238(4832) |
| Pages | 1408-10 |
| Published | 1987-12-4 |
| DOI | 10.1126/science.3500513 |
| PMID | 3500513 |
| MeSH | Animals Cell Transformation, Neoplastic / chemically induced Cell Transformation, Neoplastic / genetics* Cells, Cultured DNA, Recombinant Epidermal Growth Factor / pharmacology* ErbB Receptors / drug effects ErbB Receptors / genetics* Fibroblasts / pathology Genetic Vectors Harvey murine sarcoma virus / genetics Humans Male Mice Mice, Nude Neoplasms, Experimental / etiology Proto-Oncogene Mas Proto-Oncogenes* Recombinant Proteins / genetics |
| IF | 41.846 |
| Times Cited | 436 |
| WOS Category | BIOCHEMISTRY & MOLECULAR BIOLOGY |
| Altmetric score |
オルトメトリクス指標項目
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| The most frequently cited source | Patent(IFI CLAIMS) |
| Total number of mentions | 17 |
| Altmetric score changes over past 6months | 3.0 |
| Resource | |
| DNA material | pco12 EGFR (RDB01276) |