Reference - Detail
| RRC ID | 30987 |
|---|---|
| Author | Xie F, Murray JD, Kim J, Heckmann AB, Edwards A, Oldroyd GE, Downie JA. |
| Title | Legume pectate lyase required for root infection by rhizobia. |
| Journal | Proc Natl Acad Sci U S A |
| Abstract |
To allow rhizobial infection of legume roots, plant cell walls must be locally degraded for plant-made infection threads (ITs) to be formed. Here we identify a Lotus japonicus nodulation pectate lyase gene (LjNPL), which is induced in roots and root hairs by rhizobial nodulation (Nod) factors via activation of the nodulation signaling pathway and the NIN transcription factor. Two Ljnpl mutants produced uninfected nodules and most infections arrested as infection foci in root hairs or roots. The few partially infected nodules that did form contained large abnormal infections. The purified LjNPL protein had pectate lyase activity, demonstrating that this activity is required for rhizobia to penetrate the cell wall and initiate formation of plant-made infection threads. Therefore, we conclude that legume-determined degradation of plant cell walls is required for root infection during initiation of the symbiotic interaction between rhizobia and legumes. |
| Volume | 109(2) |
| Pages | 633-8 |
| Published | 2012-1-10 |
| DOI | 10.1073/pnas.1113992109 |
| PII | 1113992109 |
| PMID | 22203959 |
| PMC | PMC3258600 |
| MeSH | Amino Acid Sequence Base Sequence Enzyme Induction / physiology Likelihood Functions Lotus / enzymology* Lotus / growth & development Lotus / microbiology* Mesorhizobium / genetics* Mesorhizobium / metabolism Models, Genetic Molecular Sequence Data Mutation / genetics Phylogeny* Polysaccharide-Lyases / biosynthesis* Polysaccharide-Lyases / genetics* Reverse Transcriptase Polymerase Chain Reaction Root Nodules, Plant / microbiology* |
| IF | 9.412 |
| Times Cited | 111 |
| WOS Category | PLANT SCIENCES |
| Altmetric score |
オルトメトリクス指標項目
|
| The most frequently cited source | X(Twitter) |
| Total number of mentions | 12 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| Lotus / Glycine | pUB-GW-GFP |