Reference - Detail
| RRC ID | 31341 |
|---|---|
| Author | Liu T, Yamaguchi Y, Shirasaki Y, Shikada K, Yamagishi M, Hoshino K, Kaisho T, Takemoto K, Suzuki T, Kuranaga E, Ohara O, Miura M. |
| Title | Single-cell imaging of caspase-1 dynamics reveals an all-or-none inflammasome signaling response. |
| Journal | Cell Rep |
| Abstract |
Inflammasome-mediated caspase-1 activation is involved in cell death and the secretion of the proinflammatory cytokine interleukin-1β (IL-1β). Although the dynamics of caspase-1 activation, IL-1β secretion, and cell death have been examined with bulk assays in population-level studies, they remain poorly understood at the single-cell level. In this study, we conducted single-cell imaging using a genetic fluorescence resonance energy transfer sensor that detects caspase-1 activation. We determined that caspase-1 exhibits all-or-none (digital) activation at the single-cell level, with similar activation kinetics irrespective of the type of inflammasome or the intensity of the stimulus. Real-time concurrent detection of caspase-1 activation and IL-1β release demonstrated that dead macrophages containing activated caspase-1 release a local burst of IL-1β in a digital manner, which identified these macrophages as the main source of IL-1β within cell populations. Our results highlight the value of single-cell analysis in enhancing understanding of the inflammasome system and chronic inflammatory diseases. |
| Volume | 8(4) |
| Pages | 974-82 |
| Published | 2014-8-21 |
| DOI | 10.1016/j.celrep.2014.07.012 |
| PII | S2211-1247(14)00579-8 |
| PMID | 25127135 |
| MeSH | Animals Caspase 1 / physiology* Cells, Cultured Enzyme Activation Humans Inflammasomes / metabolism* Interleukin-1beta / physiology Kinetics Lipopolysaccharides / pharmacology Macrophages, Peritoneal / enzymology Macrophages, Peritoneal / immunology Mice, Transgenic Signal Transduction Single-Cell Analysis |
| IF | 8.109 |
| Times Cited | 81 |
| WOS Category | CELL BIOLOGY |
| Altmetric score |
オルトメトリクス指標項目
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| The most frequently cited source | X(Twitter) |
| Total number of mentions | 12 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| Mice | RBRC01828 |