RRC ID 31788
Author Nejigane S, Takahashi S, Haramoto Y, Michiue T, Asashima M.
Title Hippo signaling components, Mst1 and Mst2, act as a switch between self-renewal and differentiation in Xenopus hematopoietic and endothelial progenitors.
Journal Int J Dev Biol
Abstract Hippo signaling is a conserved pathway that regulates cell proliferation and organ size control. Mst1 and Mst2 were identified as homologs of hippo and as core kinases of the Hippo pathway in mammals. Here, we have characterized the role of Mst1 and Mst2 during Xenopus primitive hematopoiesis. We showed that Mst1 and Mst2 were strongly expressed in the Xenopus ventral blood island, where primitive hematopoiesis is initiated. Loss-of-function analysis of Mst1/2 revealed morphogenetic defects, including short axis, smaller eyes and abnormal epidermis, and decreased phosphorylation of Yap. Mst1/2 morphants did not exhibit any change in the expression of hematopoietic and endothelial progenitor markers in early hematopoietic development. In addition, we have shown that such progenitor markers were continuously expressed through to the late hematopoietic development stage. As a result, the expression of erythroid, myeloid and endothelial differentiation markers were decreased in Mst1/2 morphants. Our results indicate that Mst1/2 act as a differentiation switch in Xenopus hematopoietc and endothelial progenitors.
Volume 57(5)
Pages 407-14
Published 2013-1-1
DOI 10.1387/ijdb.130010st
PII 130010st
PMID 23873372
MeSH Animals Base Sequence Blotting, Western Embryo, Nonmammalian / blood supply Embryo, Nonmammalian / embryology Embryo, Nonmammalian / metabolism Endothelium / cytology Endothelium / embryology Endothelium / metabolism Erythrocytes / cytology Erythrocytes / metabolism Gene Expression Regulation, Developmental Gene Knockdown Techniques Hematopoietic System / cytology Hematopoietic System / embryology Hematopoietic System / metabolism In Situ Hybridization Molecular Sequence Data Myoblasts / cytology Myoblasts / metabolism Phosphorylation Protein-Serine-Threonine Kinases / genetics* Protein-Serine-Threonine Kinases / metabolism Reverse Transcriptase Polymerase Chain Reaction Signal Transduction / genetics* Stem Cells / metabolism* Trans-Activators / genetics Trans-Activators / metabolism Xenopus / embryology Xenopus / genetics* Xenopus Proteins / genetics* Xenopus Proteins / metabolism
IF 1.105
Times Cited 9
Xenopus tropicalis Nigerian H line