RRC ID 31789
著者 Takagi C, Sakamaki K, Morita H, Hara Y, Suzuki M, Kinoshita N, Ueno N.
タイトル Transgenic Xenopus laevis for live imaging in cell and developmental biology.
ジャーナル Dev Growth Differ
Abstract The stable transgenesis of genes encoding functional or spatially localized proteins, fused to fluorescent proteins such as green fluorescent protein (GFP) or red fluorescent protein (RFP), is an extremely important research tool in cell and developmental biology. Transgenic organisms constructed with fluorescent labels for cell membranes, subcellular organelles, and functional proteins have been used to investigate cell cycles, lineages, shapes, and polarity, in live animals and in cells or tissues derived from these animals. Genes of interest have been integrated and maintained in generations of transgenic animals, which have become a valuable resource for the cell and developmental biology communities. Although the use of Xenopus laevis as a transgenic model organism has been hampered by its relatively long reproduction time (compared to Drosophila melanogaster and Caenorhabditis elegans), its large embryonic cells and the ease of manipulation in early embryos have made it a historically valuable preparation that continues to have tremendous research potential. Here, we report on the Xenopus laevis transgenic lines our lab has generated and discuss their potential use in biological imaging.
巻・号 55(4)
ページ 422-33
公開日 2013-5-1
DOI 10.1111/dgd.12042
PMID 23480392
MeSH Animals Animals, Genetically Modified* Apoptosis Cell Membrane / metabolism Cell Nucleus / metabolism Developmental Biology / methods* Gene Expression Regulation, Developmental Genetic Techniques Green Fluorescent Proteins / metabolism Luminescent Proteins / metabolism Microtubules / metabolism Promoter Regions, Genetic Transgenes Whole Body Imaging* Xenopus laevis / genetics*
IF 1.723
引用数 27
WOS 分野 DEVELOPMENTAL BIOLOGY CELL BIOLOGY
リソース情報
ツメガエル・イモリ Golden line