RRC ID 31792
Author Miyake A, Araki M.
Title Retinal stem/progenitor cells in the ciliary marginal zone complete retinal regeneration: a study of retinal regeneration in a novel animal model.
Journal Dev Neurobiol
Abstract Our research group has extensively studied retinal regeneration in adult Xenopus laevis. However, X. laevis does not represent a suitable model for multigenerational genetics and genomic approaches. Instead, Xenopus tropicalis is considered as the ideal model for these studies, although little is known about retinal regeneration in X. tropicalis. In the present study, we showed that a complete retina regenerates at approximately 30 days after whole retinal removal. The regenerating retina was derived from the stem/progenitor cells in the ciliary marginal zone (CMZ), indicating a novel mode of vertebrate retinal regeneration, which has not been previously reported. In a previous study, we showed that in X. laevis, retinal regeneration occurs primarily through the transdifferentiation of retinal pigmented epithelial (RPE) cells. RPE cells migrate to the retinal vascular membrane and reform a new epithelium, which then differentiates into the retina. In X. tropicalis, RPE cells also migrated to the vascular membrane, but transdifferentiation was not evident. Using two tissue culture models of RPE tissues, it was shown that in X. laevis RPE culture neuronal differentiation and reconstruction of the retinal three-dimensional (3-D) structure were clearly observed, while in X. tropicalis RPE culture neither ßIII tubulin-positive cells nor 3-D retinal structure were seen. These results indicate that the two Xenopus species are excellent models to clarify the cellular and molecular mechanisms of retinal regeneration, as these animals have contrasting modes of regeneration; one mode primarily involves RPE cells and the other mode involves stem/progenitor cells in the CMZ.
Volume 74(7)
Pages 739-56
Published 2014-7-1
DOI 10.1002/dneu.22169
PMID 24488715
MeSH Animals Apoptosis / physiology Cell Count Cell Movement / physiology Cell Transdifferentiation / physiology Immunohistochemistry Models, Animal Nerve Regeneration / physiology Neurogenesis / physiology Regeneration / physiology* Retina / growth & development Retina / physiology* Retina / surgery Retinal Pigment Epithelium / physiology Species Specificity Stem Cells / physiology Tissue Culture Techniques Tubulin / metabolism Xenopus / growth & development Xenopus / physiology* Xenopus Proteins / metabolism Xenopus laevis / growth & development Xenopus laevis / physiology
IF 3.935
Times Cited 22
WOS Category DEVELOPMENTAL BIOLOGY NEUROSCIENCES
Resource
Xenopus tropicalis Nigerian H line