RRC ID 31879
Author Murakami-Tonami Y, Ohtsuka H, Aiba H, Murakami H.
Title Regulation of wee1(+) expression during meiosis in fission yeast.
Journal Cell Cycle
Abstract In eukaryotes, the cyclin-dependent kinase Cdk1p (Cdc2p) plays a central role in entry into and progression through nuclear division during mitosis and meiosis. Cdk1p is activated during meiotic nuclear divisions by dephosphorylation of its tyrosine-15 residue. The phosphorylation status of this residue is largely determined by the Wee1p kinase and the Cdc25p phosphatase. In fission yeast, the forkhead-type transcription factor Mei4p is essential for entry into the first meiotic nuclear division. We recently identified cdc25(+) as an essential target of Mei4p in the control of entry into meiosis I. Here, we show that wee1(+) is another important target of Mei4p in the control of entry into meiosis I. Mei4p bound to the upstream region of wee1(+) in vivo and in vitro and inhibited expression of wee1(+), whereas Mei4p positively regulated expression of the adjacent pseudogene. Overexpression of Mei4p inhibited expression of wee1(+) and induced that of the pseudogene. Conversely, deletion of Mei4p did not decrease expression of wee1(+) but inhibited that of the pseudogene. In addition, deletion of Mei4p-binding regions delayed repression of wee1(+) expression as well as induction of expression of the pseudogene. These results suggest that repression of wee1(+) expression is primarily owing to Mei4p-mediated transcriptional interference.
Volume 13(18)
Pages 2853-8
Published 2014
DOI 10.4161/15384101.2014.946807
PMID 25486473
PMC PMC4612672
MeSH Base Sequence Cell Cycle Proteins / genetics Cell Cycle Proteins / metabolism* Gene Deletion Gene Expression Regulation, Fungal* Genes, Fungal Meiosis / genetics* Mitosis Models, Biological Molecular Sequence Data Nuclear Proteins / genetics Nuclear Proteins / metabolism* Protein Binding Protein-Tyrosine Kinases / genetics Protein-Tyrosine Kinases / metabolism* Pseudogenes RNA, Messenger / genetics RNA, Messenger / metabolism Regulatory Sequences, Nucleic Acid / genetics Schizosaccharomyces / cytology* Schizosaccharomyces / genetics* Schizosaccharomyces pombe Proteins / genetics Schizosaccharomyces pombe Proteins / metabolism* Transcription, Genetic
IF 3.259
Times Cited 0
Yeast FY16057(HM1307) FY25243(HM2163) FY25244(HM4582) FY25245(HM4732) FY25246(HM4735) FY25247(HM4833) FY25248(HM5678)