論文 - 詳細
| RRC ID | 31879 |
|---|---|
| 著者 | Murakami-Tonami Y, Ohtsuka H, Aiba H, Murakami H. |
| タイトル | Regulation of wee1(+) expression during meiosis in fission yeast. |
| ジャーナル | Cell Cycle |
| Abstract |
In eukaryotes, the cyclin-dependent kinase Cdk1p (Cdc2p) plays a central role in entry into and progression through nuclear division during mitosis and meiosis. Cdk1p is activated during meiotic nuclear divisions by dephosphorylation of its tyrosine-15 residue. The phosphorylation status of this residue is largely determined by the Wee1p kinase and the Cdc25p phosphatase. In fission yeast, the forkhead-type transcription factor Mei4p is essential for entry into the first meiotic nuclear division. We recently identified cdc25(+) as an essential target of Mei4p in the control of entry into meiosis I. Here, we show that wee1(+) is another important target of Mei4p in the control of entry into meiosis I. Mei4p bound to the upstream region of wee1(+) in vivo and in vitro and inhibited expression of wee1(+), whereas Mei4p positively regulated expression of the adjacent pseudogene. Overexpression of Mei4p inhibited expression of wee1(+) and induced that of the pseudogene. Conversely, deletion of Mei4p did not decrease expression of wee1(+) but inhibited that of the pseudogene. In addition, deletion of Mei4p-binding regions delayed repression of wee1(+) expression as well as induction of expression of the pseudogene. These results suggest that repression of wee1(+) expression is primarily owing to Mei4p-mediated transcriptional interference. |
| 巻・号 | 13(18) |
| ページ | 2853-8 |
| 公開日 | 2014-1-1 |
| DOI | 10.4161/15384101.2014.946807 |
| PMID | 25486473 |
| PMC | PMC4612672 |
| MeSH | Base Sequence Cell Cycle Proteins / genetics Cell Cycle Proteins / metabolism* Gene Deletion Gene Expression Regulation, Fungal* Genes, Fungal Meiosis / genetics* Mitosis Models, Biological Molecular Sequence Data Nuclear Proteins / genetics Nuclear Proteins / metabolism* Protein Binding Protein-Tyrosine Kinases / genetics Protein-Tyrosine Kinases / metabolism* Pseudogenes RNA, Messenger / genetics RNA, Messenger / metabolism Regulatory Sequences, Nucleic Acid / genetics Schizosaccharomyces / cytology* Schizosaccharomyces / genetics* Schizosaccharomyces pombe Proteins / genetics Schizosaccharomyces pombe Proteins / metabolism* Transcription, Genetic |
| IF | 3.699 |
| 引用数 | 1 |
| WOS 分野 | CELL BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 3 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 酵母 | FY16057(HM1307) FY25243(HM2163) FY25244(HM4582) FY25245(HM4732) FY25246(HM4735) FY25247(HM4833) FY25248(HM5678) |