| RRC ID |
31879
|
| 著者 |
Murakami-Tonami Y, Ohtsuka H, Aiba H, Murakami H.
|
| タイトル |
Regulation of wee1(+) expression during meiosis in fission yeast.
|
| ジャーナル |
Cell Cycle
|
| Abstract |
In eukaryotes, the cyclin-dependent kinase Cdk1p (Cdc2p) plays a central role in entry into and progression through nuclear division during mitosis and meiosis. Cdk1p is activated during meiotic nuclear divisions by dephosphorylation of its tyrosine-15 residue. The phosphorylation status of this residue is largely determined by the Wee1p kinase and the Cdc25p phosphatase. In fission yeast, the forkhead-type transcription factor Mei4p is essential for entry into the first meiotic nuclear division. We recently identified cdc25(+) as an essential target of Mei4p in the control of entry into meiosis I. Here, we show that wee1(+) is another important target of Mei4p in the control of entry into meiosis I. Mei4p bound to the upstream region of wee1(+) in vivo and in vitro and inhibited expression of wee1(+), whereas Mei4p positively regulated expression of the adjacent pseudogene. Overexpression of Mei4p inhibited expression of wee1(+) and induced that of the pseudogene. Conversely, deletion of Mei4p did not decrease expression of wee1(+) but inhibited that of the pseudogene. In addition, deletion of Mei4p-binding regions delayed repression of wee1(+) expression as well as induction of expression of the pseudogene. These results suggest that repression of wee1(+) expression is primarily owing to Mei4p-mediated transcriptional interference.
|
| 巻・号 |
13(18)
|
| ページ |
2853-8
|
| 公開日 |
2014-1-1
|
| DOI |
10.4161/15384101.2014.946807
|
| PMID |
25486473
|
| PMC |
PMC4612672
|
| MeSH |
Base Sequence
Cell Cycle Proteins / genetics
Cell Cycle Proteins / metabolism*
Gene Deletion
Gene Expression Regulation, Fungal*
Genes, Fungal
Meiosis / genetics*
Mitosis
Models, Biological
Molecular Sequence Data
Nuclear Proteins / genetics
Nuclear Proteins / metabolism*
Protein Binding
Protein-Tyrosine Kinases / genetics
Protein-Tyrosine Kinases / metabolism*
Pseudogenes
RNA, Messenger / genetics
RNA, Messenger / metabolism
Regulatory Sequences, Nucleic Acid / genetics
Schizosaccharomyces / cytology*
Schizosaccharomyces / genetics*
Schizosaccharomyces pombe Proteins / genetics
Schizosaccharomyces pombe Proteins / metabolism*
Transcription, Genetic
|
| IF |
3.699
|
| 引用数 |
1
|
|
WOS 分野
|
CELL BIOLOGY
|
| リソース情報 |
| 酵母 |
FY16057(HM1307)
FY25243(HM2163)
FY25244(HM4582)
FY25245(HM4732)
FY25246(HM4735)
FY25247(HM4833)
FY25248(HM5678) |
