RRC ID 31989
Author Aomatsu E, Takahashi N, Sawada S, Okubo N, Hasegawa T, Taira M, Miura H, Ishisaki A, Chosa N.
Title Novel SCRG1/BST1 axis regulates self-renewal, migration, and osteogenic differentiation potential in mesenchymal stem cells.
Journal Sci Rep
Abstract Human mesenchymal stem cells (hMSCs) remodel or regenerate various tissues through several mechanisms. Here, we identified the hMSC-secreted protein SCRG1 and its receptor BST1 as a positive regulator of self-renewal, migration, and osteogenic differentiation. SCRG1 and BST1 gene expression decreased during osteogenic differentiation of hMSCs. Intriguingly, SCRG1 maintained stem cell marker expression (Oct-4 and CD271/LNGFR) and the potentials of self-renewal, migration, and osteogenic differentiation, even at high passage numbers. Thus, the novel SCRG1/BST1 axis determines the fate of hMSCs by regulating their kinetic and differentiation potentials. Our findings provide a new perspective on methods for ex vivo expansion of hMSCs that maintain native stem cell potentials for bone-forming cell therapy.
Volume 4
Pages 3652
Published 2014-1-13
DOI 10.1038/srep03652
PII srep03652
PMID 24413464
PMC PMC3888969
MeSH ADP-ribosyl Cyclase / genetics* ADP-ribosyl Cyclase / metabolism Antigens, CD / genetics* Antigens, CD / metabolism Cell Differentiation / genetics* Cell Movement / genetics Down-Regulation Extracellular Signal-Regulated MAP Kinases / metabolism Focal Adhesion Protein-Tyrosine Kinases / metabolism GPI-Linked Proteins / genetics GPI-Linked Proteins / metabolism Gene Expression Regulation, Developmental Humans JNK Mitogen-Activated Protein Kinases / metabolism Mesenchymal Stem Cells / cytology Mesenchymal Stem Cells / metabolism* Nerve Tissue Proteins / genetics* Nerve Tissue Proteins / metabolism Osteogenesis / genetics* Phosphatidylinositol 3-Kinases / metabolism Protein Binding Protein Biosynthesis Signal Transduction
IF 3.998
Times Cited 28
DNA material pSCRG1-FLAG (RDB12800)