論文 - 詳細
| RRC ID | 3207 |
|---|---|
| 著者 | Kanehara K, Ito K, Akiyama Y. |
| タイトル | YaeL proteolysis of RseA is controlled by the PDZ domain of YaeL and a Gln-rich region of RseA. |
| ジャーナル | EMBO J |
| Abstract |
sigmaE is an alternative sigma factor involved in a pathway of extracytoplasmic stress responses in Escherichia coli. Under normal growth conditions, sigmaE activity is down-regulated by the membrane-bound anti-sigmaE protein, RseA. Extracytoplasmic stress signals induce degradation of RseA by two successive proteolytic events: DegS-catalyzed first cleavage at a periplasmic site followed by YaeL-mediated second proteolysis at an intramembrane region. Normally, the second reaction (site-2 proteolysis) only occurs after the first cleavage (site-1 cleavage). Here, we show that YaeL variants with the periplasmic PDZ domain deleted or mutated allows unregulated cleavage of RseA and consequent sigmaE activation. It was also found that a glutamine-rich region in the periplasmic domain of RseA was required for the avoidance of the YaeL-mediated proteolysis in the absence of site-1 cleavage. These results indicate that multiple negative elements both in the enzyme (PDZ domain) and in the substrate (glutamine-rich region) determine the strict dependence of the site-2 proteolysis on the site-1 cleavage. |
| 巻・号 | 22(23) |
| ページ | 6389-98 |
| 公開日 | 2003-12-1 |
| DOI | 10.1093/emboj/cdg602 |
| PMID | 14633997 |
| PMC | PMC291843 |
| MeSH | Amino Acid Sequence Binding Sites Endopeptidases / chemistry* Endopeptidases / metabolism* Escherichia coli Proteins / chemistry* Escherichia coli Proteins / metabolism* Genetic Complementation Test Glutamine* Kinetics Membrane Proteins / chemistry* Membrane Proteins / metabolism* Molecular Sequence Data Mutagenesis Plasmids Recombinant Proteins / chemistry Recombinant Proteins / metabolism Sequence Alignment Sequence Homology, Amino Acid Transcription Factors / chemistry* Transcription Factors / metabolism* |
| IF | 9.889 |
| 引用数 | 81 |
| WOS 分野 | BIOCHEMISTRY & MOLECULAR BIOLOGY CELL BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 各媒体での言及数の合計 | 0 |
| リソース情報 | |
| 原核生物(大腸菌) | |