論文 - 詳細
| RRC ID | 32178 |
|---|---|
| 著者 | Kita A, Higa M, Doi A, Satoh R, Sugiura R. |
| タイトル | Imp2, the PSTPIP homolog in fission yeast, affects sensitivity to the immunosuppressant FK506 and membrane trafficking in fission yeast. |
| ジャーナル | Biochem Biophys Res Commun |
| Abstract |
Cytokinesis is a highly ordered process that divides one cell into two cells, which is functionally linked to the dynamic remodeling of the plasma membrane coordinately with various events such as membrane trafficking. Calcineurin is a highly conserved serine/threonine protein phosphatase, which regulates multiple biological functions, such as membrane trafficking and cytokinesis. Here, we isolated imp2-c3, a mutant allele of the imp2(+) gene, encoding a homolog of the mouse PSTPIP1 (proline-serine-threonine phosphatase interacting protein 1), using a genetic screen for mutations that are synthetically lethal with calcineurin deletion in fission yeast. The imp2-c3 mutants showed a defect in cytokinesis with multi-septated phenotypes, which was further enhanced upon treatment with the calcineurin inhibitor FK506. Notably, electron micrographs revealed that the imp2-c3 mutant cells accumulated aberrant multi-lamella Golgi structures and putative post-Golgi secretory vesicles, and exhibited fragmented vacuoles in addition to thickened septa. Consistently, imp2-c3 mutants showed a reduced secretion of acid phosphatase and defects in vacuole fusion. The imp2-c3 mutant cells exhibited a weakened cell wall, similar to the membrane trafficking mutants identified in the same genetic screen such as ypt3-i5. These findings implicate the PSTPIP1 homolog Imp2 in Golgi/vacuole function, thereby affecting various cellular processes, including cytokinesis and cell integrity. |
| 巻・号 | 457(3) |
| ページ | 273-9 |
| 公開日 | 2015-2-13 |
| DOI | 10.1016/j.bbrc.2014.12.100 |
| PII | S0006-291X(14)02289-X |
| PMID | 25580011 |
| MeSH | Adaptor Proteins, Signal Transducing / genetics Animals Cell Wall / metabolism Cell Wall / ultrastructure Cloning, Molecular Cytokinesis / drug effects Cytokinesis / genetics Cytoskeletal Proteins / genetics Cytoskeletal Proteins / metabolism* Genes, Fungal Immunosuppressive Agents / pharmacology Mice Microscopy, Electron, Transmission Mutation Schizosaccharomyces / drug effects Schizosaccharomyces / genetics Schizosaccharomyces / metabolism* Schizosaccharomyces pombe Proteins / genetics Schizosaccharomyces pombe Proteins / metabolism* Tacrolimus / pharmacology Vacuoles / metabolism Vacuoles / ultrastructure |
| IF | 2.985 |
| 引用数 | 3 |
| WOS 分野 | BIOPHYSICS BIOCHEMISTRY & MOLECULAR BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 2 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 酵母 | FY9899? |