Reference - Detail
| RRC ID | 32213 |
|---|---|
| Author | Lorenzi LE, Bah A, Wischnewski H, Shchepachev V, Soneson C, Santagostino M, Azzalin CM. |
| Title | Fission yeast Cactin restricts telomere transcription and elongation by controlling Rap1 levels. |
| Journal | EMBO J |
| Abstract |
The telomeric transcriptome comprises multiple long non-coding RNAs generated by transcription of linear chromosome ends. In a screening performed in Schizosaccharomyces pombe, we identified factors modulating the cellular levels of the telomeric transcriptome. Among these factors, Cay1 is the fission yeast member of the conserved family of Cactins, uncharacterized proteins crucial for cell growth and survival. In cay1∆ mutants, the cellular levels of the telomeric factor Rap1 are drastically diminished due to defects in rap1+ pre-mRNA splicing and Rap1 protein stability. cay1∆ cells accumulate histone H3 acetylated at lysine 9 at telomeres, which become transcriptionally desilenced, are over-elongated by telomerase and cause chromosomal aberrations in the cold. Overexpressing Rap1 in cay1+ deleted cells significantly reverts all telomeric defects. Additionally, cay1∆ mutants accumulate unprocessed Tf2 retrotransposon RNA through Rap1-independent mechanisms. Thus, Cay1 plays crucial roles in cells by ultimately harmonizing expression of transcripts originating from seemingly unrelated genomic loci. |
| Volume | 34(1) |
| Pages | 115-29 |
| Published | 2015-1-2 |
| DOI | 10.15252/embj.201489559 |
| PII | embj.201489559 |
| PMID | 25398909 |
| PMC | PMC4291484 |
| MeSH | Chromosome Aberrations Chromosomes, Fungal / genetics Chromosomes, Fungal / metabolism* Gene Deletion Nuclear Proteins / genetics Nuclear Proteins / metabolism* Protein Stability RNA Splicing / physiology RNA, Fungal / genetics RNA, Fungal / metabolism RNA, Messenger / genetics RNA, Messenger / metabolism Retroelements / physiology Schizosaccharomyces / genetics Schizosaccharomyces / metabolism* Schizosaccharomyces pombe Proteins / genetics Schizosaccharomyces pombe Proteins / metabolism Shelterin Complex Telomere / genetics Telomere / metabolism* Telomere-Binding Proteins / genetics Telomere-Binding Proteins / metabolism Transcription, Genetic / physiology* |
| IF | 9.889 |
| Times Cited | 12 |
| WOS Category | BIOCHEMISTRY & MOLECULAR BIOLOGY CELL BIOLOGY |
| Altmetric score |
オルトメトリクス指標項目
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| The most frequently cited source | X(Twitter) |
| Total number of mentions | 6 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| DNA material | S. pombe YFP-FLAG-His plasmid SpYFH27C12 (SPW090860) SpYFH26G09 (SPW090553) SpYFH36C12 (SPW094460). |