RRC ID 32230
Author Murata T, Ishitsuka Y, Karouji K, Kaneda H, Toki H, Nakai Y, Makino S, Fukumura R, Kotaki H, Wakana S, Noda T, Gondo Y.
Title β-CateninC429S mice exhibit sterility consequent to spatiotemporally sustained Wnt signalling in the internal genitalia.
Journal Sci Rep
Abstract Wnt/β-catenin signalling regulates numerous developmental and homeostatic processes. Ctnnb1 (also known as β-catenin) is the only protein that transmits signals from various Wnt ligands to downstream genes. In this study, we report that our newly established mouse strain, which harbours a Cys429 to Ser missense mutation in the β-catenin gene, exhibited specific organ defects in contrast to mice with broadly functioning Wnt/β-catenin signalling. Both homozygous mutant males and females produced normal gametes but were infertile because of abnormal seminal vesicle and vaginal morphogenesis. An ins-TOPGAL transgenic reporter spatiotemporally sustained Wnt/β-catenin signalling during the corresponding organogenesis. Therefore, β-catenin(C429S) should provide new insights into β-catenin as a universal component of Wnt/β-catenin signal transduction.
Volume 4
Pages 6959
Published 2014-11-7
DOI 10.1038/srep06959
PII srep06959
PMID 25376241
PMC PMC4223658
MeSH Animals Embryo, Mammalian Female Genes, Reporter Homozygote Infertility, Female / genetics* Infertility, Male / genetics* Male Mice Mice, Inbred C57BL Mice, Transgenic Mutation* Ovum / growth & development Ovum / metabolism Seminal Vesicles / abnormalities Seminal Vesicles / growth & development Seminal Vesicles / metabolism* Spermatozoa / growth & development Spermatozoa / metabolism Vagina / abnormalities Vagina / growth & development Vagina / metabolism* Wnt Proteins / genetics Wnt Proteins / metabolism Wnt Signaling Pathway* beta Catenin / genetics* beta Catenin / metabolism
IF 3.998
Mice RBRC02228