RRC ID 32469
著者 Babaoğlan AB, Housden BE, Furriols M, Bray SJ.
タイトル Deadpan contributes to the robustness of the notch response.
ジャーナル PLoS One
Abstract Notch signaling regulates many fundamental events including lateral inhibition and boundary formation to generate very reproducible patterns in developing tissues. Its targets include genes of the bHLH hairy and Enhancer of split [E(spl)] family, which contribute to many of these developmental decisions. One member of this family in Drosophila, deadpan (dpn), was originally found to have functions independent of Notch in promoting neural development. Employing genome-wide chromatin-immunoprecipitation we have identified several Notch responsive enhancers in dpn, demonstrating its direct regulation by Notch in a range of contexts including the Drosophila wing and eye. dpn expression largely overlaps that of several E(spl) genes and the combined knock-down leads to more severe phenotypes than either alone. In addition, Dpn contributes to the establishment of Cut expression at the wing dorsal-ventral (D/V) boundary; in its absence Cut expression is delayed. Furthermore, over-expression of Dpn inhibits expression from E(spl) gene enhancers, but not vice versa, suggesting that dpn contributes to a feed-back mechanism that limits E(spl) gene expression following Notch activation. Thus the combined actions of dpn and E(spl) appear to provide a mechanism that confers an initial rapid output from Notch activity which becomes self-limited via feedback between the targets.
巻・号 8(9)
ページ e75632
公開日 2013-9-24
DOI 10.1371/journal.pone.0075632
PII PONE-D-13-24754
PMID 24086596
PMC PMC3782438
MeSH Animals Basic Helix-Loop-Helix Transcription Factors / genetics* Basic Helix-Loop-Helix Transcription Factors / metabolism* DNA-Binding Proteins Drosophila / genetics Drosophila / growth & development* Drosophila / metabolism Drosophila Proteins / genetics* Drosophila Proteins / metabolism* Eye / growth & development Eye / metabolism Gene Expression Regulation, Developmental / genetics Homeodomain Proteins / genetics Homeodomain Proteins / metabolism Nuclear Proteins / genetics* Nuclear Proteins / metabolism* Receptors, Notch / genetics* Receptors, Notch / metabolism* Repressor Proteins / genetics Repressor Proteins / metabolism Transcription Factors / genetics Transcription Factors / metabolism Wings, Animal / growth & development Wings, Animal / metabolism
IF 2.74
引用数 10
WOS 分野 DEVELOPMENTAL BIOLOGY
リソース情報
ショウジョウバエ 8704R-4