RRC ID 32504
著者 Vagin VV, Yu Y, Jankowska A, Luo Y, Wasik KA, Malone CD, Harrison E, Rosebrock A, Wakimoto BT, Fagegaltier D, Muerdter F, Hannon GJ.
タイトル Minotaur is critical for primary piRNA biogenesis.
ジャーナル RNA
Abstract Piwi proteins and their associated small RNAs are essential for fertility in animals. In part, this is due to their roles in guarding germ cell genomes against the activity of mobile genetic elements. piRNA populations direct Piwi proteins to silence transposon targets and, as such, form a molecular code that discriminates transposons from endogenous genes. Information ultimately carried by piRNAs is encoded within genomic loci, termed piRNA clusters. These give rise to long, single-stranded, primary transcripts that are processed into piRNAs. Despite the biological importance of this pathway, neither the characteristics that define a locus as a source of piRNAs nor the mechanisms that catalyze primary piRNA biogenesis are well understood. We searched an EMS-mutant collection annotated for fertility phenotypes for genes involved in the piRNA pathway. Twenty-seven homozygous sterile strains showed transposon-silencing defects. One of these, which strongly impacted primary piRNA biogenesis, harbored a causal mutation in CG5508, a member of the Drosophila glycerol-3-phosphate O-acetyltransferase (GPAT) family. These enzymes catalyze the first acylation step on the path to the production of phosphatidic acid (PA). Though this pointed strongly to a function for phospholipid signaling in the piRNA pathway, a mutant form of CG5508, which lacks the GPAT active site, still functions in piRNA biogenesis. We have named this new biogenesis factor Minotaur.
巻・号 19(8)
ページ 1064-77
公開日 2013-8-1
DOI 10.1261/rna.039669.113
PII rna.039669.113
PMID 23788724
PMC PMC3708527
MeSH Amino Acid Sequence Animals Animals, Genetically Modified Argonaute Proteins / genetics Argonaute Proteins / metabolism Catalytic Domain DNA Transposable Elements / genetics Drosophila Proteins / genetics Drosophila Proteins / metabolism* Drosophila melanogaster / genetics Drosophila melanogaster / metabolism* Female Genes, Insect Glycerol-3-Phosphate O-Acyltransferase / genetics Glycerol-3-Phosphate O-Acyltransferase / metabolism* Male Molecular Sequence Data Mutation Phospholipids / metabolism RNA, Small Interfering / biosynthesis* RNA, Small Interfering / genetics Signal Transduction
IF 4.32
引用数 34
WOS 分野 BIOCHEMISTRY & MOLECULAR BIOLOGY
リソース情報
ショウジョウバエ DGRC#109171