論文 - 詳細
| RRC ID | 32512 |
|---|---|
| 著者 | Charng WL, Yamamoto S, Jaiswal M, Bayat V, Xiong B, Zhang K, Sandoval H, David G, Gibbs S, Lu HC, Chen K, Giagtzoglou N, Bellen HJ. |
| タイトル | Drosophila Tempura, a novel protein prenyltransferase α subunit, regulates notch signaling via Rab1 and Rab11. |
| ジャーナル | PLoS Biol |
| Abstract |
Vesicular trafficking plays a key role in tuning the activity of Notch signaling. Here, we describe a novel and conserved Rab geranylgeranyltransferase (RabGGT)-α-like subunit that is required for Notch signaling-mediated lateral inhibition and cell fate determination of external sensory organs. This protein is encoded by tempura, and its loss affects the secretion of Scabrous and Delta, two proteins required for proper Notch signaling. We show that Tempura forms a heretofore uncharacterized RabGGT complex that geranylgeranylates Rab1 and Rab11. This geranylgeranylation is required for their proper subcellular localization. A partial dysfunction of Rab1 affects Scabrous and Delta in the secretory pathway. In addition, a partial loss Rab11 affects trafficking of Delta. In summary, Tempura functions as a new geranylgeranyltransferase that regulates the subcellular localization of Rab1 and Rab11, which in turn regulate trafficking of Scabrous and Delta, thereby affecting Notch signaling. |
| 巻・号 | 12(1) |
| ページ | e1001777 |
| 公開日 | 2014-1-1 |
| DOI | 10.1371/journal.pbio.1001777 |
| PII | PBIOLOGY-D-13-02923 |
| PMID | 24492843 |
| PMC | PMC3904817 |
| MeSH | Animals Dimethylallyltranstransferase / genetics* Dimethylallyltranstransferase / metabolism Drosophila Proteins / genetics* Drosophila Proteins / metabolism Drosophila melanogaster / genetics* Drosophila melanogaster / metabolism Gene Expression Regulation Glycoproteins / genetics Glycoproteins / metabolism Intracellular Signaling Peptides and Proteins / genetics Intracellular Signaling Peptides and Proteins / metabolism Membrane Proteins / genetics Membrane Proteins / metabolism Protein Prenylation Protein Processing, Post-Translational* Protein Subunits / genetics* Protein Subunits / metabolism Receptors, Notch / genetics* Receptors, Notch / metabolism Signal Transduction rab GTP-Binding Proteins / genetics* rab GTP-Binding Proteins / metabolism rab1 GTP-Binding Proteins / genetics* rab1 GTP-Binding Proteins / metabolism |
| IF | 7.076 |
| 引用数 | 30 |
| WOS 分野 | BIOLOGY BIOCHEMISTRY & MOLECULAR BIOLOGY |
| リソース情報 | |
| ショウジョウバエ | |