RRC ID 32516
著者 Hamada-Kawaguchi N, Nore BF, Kuwada Y, Smith CI, Yamamoto D.
タイトル Btk29A promotes Wnt4 signaling in the niche to terminate germ cell proliferation in Drosophila.
ジャーナル Science
Abstract Btk29A is the Drosophila ortholog of the mammalian Bruton's tyrosine kinase (Btk), mutations of which in humans cause a heritable immunodeficiency disease. Btk29A mutations stabilized the proliferating cystoblast fate, leading to an ovarian tumor. This phenotype was rescued by overexpression of wild-type Btk29A and phenocopied by the interference of Wnt4-β-catenin signaling or its putative downstream nuclear protein Piwi in somatic escort cells. Btk29A and mammalian Btk directly phosphorylated tyrosine residues of β-catenin, leading to the up-regulation of its transcriptional activity. Thus, we identify a transcriptional switch involving the kinase Btk29A/Btk and its phosphorylation target, β-catenin, which functions downstream of Wnt4 in escort cells to terminate Drosophila germ cell proliferation through up-regulation of piwi expression. This signaling mechanism likely represents a versatile developmental switch.
巻・号 343(6168)
ページ 294-7
公開日 2014-1-17
DOI 10.1126/science.1244512
PII 343/6168/294
PMID 24436419
MeSH Animals Argonaute Proteins / biosynthesis* Cell Proliferation* DNA Breaks, Double-Stranded Drosophila Proteins / biosynthesis* Drosophila Proteins / genetics Drosophila Proteins / metabolism* Drosophila melanogaster / genetics Drosophila melanogaster / metabolism Drosophila melanogaster / physiology* Gene Knockdown Techniques Genomic Instability Germ Cells / cytology Germ Cells / metabolism Germ Cells / physiology* Glycoproteins / genetics Glycoproteins / metabolism* Phosphorylation Protein-Tyrosine Kinases / genetics Protein-Tyrosine Kinases / metabolism* RNA, Small Interfering / genetics RNA, Small Interfering / metabolism Signal Transduction Transcription, Genetic Tyrosine / genetics Tyrosine / metabolism Up-Regulation Wnt Proteins / genetics Wnt Proteins / metabolism* beta Catenin / genetics beta Catenin / metabolism*
IF 41.846
引用数 33
WOS 分野 CELL BIOLOGY
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