Reference - Detail
| RRC ID | 32530 |
|---|---|
| Author | Shields AR, Spence AC, Yamashita YM, Davies EL, Fuller MT. |
| Title | The actin-binding protein profilin is required for germline stem cell maintenance and germ cell enclosure by somatic cyst cells. |
| Journal | Development |
| Abstract |
Specialized microenvironments, or niches, provide signaling cues that regulate stem cell behavior. In the Drosophila testis, the JAK-STAT signaling pathway regulates germline stem cell (GSC) attachment to the apical hub and somatic cyst stem cell (CySC) identity. Here, we demonstrate that chickadee, the Drosophila gene that encodes profilin, is required cell autonomously to maintain GSCs, possibly facilitating localization or maintenance of E-cadherin to the GSC-hub cell interface. Germline specific overexpression of Adenomatous Polyposis Coli 2 (APC2) rescued GSC loss in chic hypomorphs, suggesting an additive role of APC2 and F-actin in maintaining the adherens junctions that anchor GSCs to the niche. In addition, loss of chic function in the soma resulted in failure of somatic cyst cells to maintain germ cell enclosure and overproliferation of transit-amplifying spermatogonia. |
| Volume | 141(1) |
| Pages | 73-82 |
| Published | 2014-1-1 |
| DOI | 10.1242/dev.101931 |
| PII | 141/1/73 |
| PMID | 24346697 |
| PMC | PMC3865751 |
| MeSH | Actins / metabolism Adherens Junctions / metabolism Animals Cadherins / metabolism Cell Differentiation Cell Lineage Cell Proliferation Drosophila Proteins / biosynthesis Drosophila Proteins / genetics Drosophila Proteins / metabolism* Drosophila melanogaster / cytology Drosophila melanogaster / metabolism* Gene Expression Regulation, Developmental Male Profilins / genetics Profilins / metabolism* Protein Binding RNA Interference RNA, Small Interfering / genetics Signal Transduction / genetics Spermatogonia / cytology Spermatogonia / metabolism Spermatozoa / cytology Spermatozoa / metabolism* Stem Cell Niche Stem Cells / metabolism* Tumor Suppressor Proteins / biosynthesis Tumor Suppressor Proteins / metabolism* |
| IF | 5.611 |
| Times Cited | 23 |
| WOS Category | DEVELOPMENTAL BIOLOGY |
| Resource | |
| Drosophila | UAS-DEFL#6-1 i.e. UAS-Ecadherin-GFP (DGRC#109005) |