論文 - 詳細
| RRC ID | 32587 |
|---|---|
| 著者 | Kayagaki N, Yamaguchi N, Nakayama M, Takeda K, Akiba H, Tsutsui H, Okamura H, Nakanishi K, Okumura K, Yagita H. |
| タイトル | Expression and function of TNF-related apoptosis-inducing ligand on murine activated NK cells. |
| ジャーナル | J Immunol |
| Abstract |
TNF-related apoptosis-inducing ligand (TRAIL), a new member of TNF family, induces apoptotic cell death of various tumor cells. We recently showed that TRAIL mediates perforin- and Fas ligand (FasL)-independent cytotoxic activity of human CD4+ T cell clones. In the present study, we investigated the expression and function of TRAIL on murine lymphocytes by using newly generated anti-murine TRAIL mAbs. Although freshly isolated T, B, or NK cells did not express a detectable level of TRAIL on their surface, a remarkable level of TRAIL expression was induced preferentially on CD3- NK1.1+ NK cells after stimulation with IL-2 or IL-15. In contrast, TRAIL expression was not induced by IL-18, whereas it efficiently potentiated lymphokine-activated killer activity of NK cells. In addition to perforin inactivation and neutralization of FasL by anti-FasL mAb, neutralization of TRAIL by anti-TRAIL mAb was needed for the complete inhibition of IL-2- or IL-15-activated NK cell cytotoxicity against mouse fibrosarcoma L929 target cells, which were susceptible to both FasL and TRAIL. These results indicated preferential expression of TRAIL on IL-2- or IL-15-activated NK cells and its potential involvement in lymphokine-activated killer activity. |
| 巻・号 | 163(4) |
| ページ | 1906-13 |
| 公開日 | 1999-8-15 |
| PII | ji_v163n4p1906 |
| PMID | 10438925 |
| MeSH | Animals Antibodies, Monoclonal / chemistry Antibodies, Monoclonal / metabolism Apoptosis / genetics Apoptosis / immunology* Apoptosis Regulatory Proteins Cell Line Cytotoxicity, Immunologic Humans Interleukin-15 / pharmacology Interleukin-2 / pharmacology Killer Cells, Natural / immunology* Killer Cells, Natural / metabolism* Ligands Lymphocyte Activation* / genetics Male Membrane Glycoproteins / biosynthesis* Membrane Glycoproteins / genetics Membrane Glycoproteins / immunology Membrane Glycoproteins / metabolism Mice Mice, Inbred C57BL Rats Rats, Inbred F344 Recombinant Proteins / biosynthesis Recombinant Proteins / chemistry Recombinant Proteins / immunology Recombinant Proteins / metabolism TNF-Related Apoptosis-Inducing Ligand Transfection Tumor Cells, Cultured Tumor Necrosis Factor-alpha / biosynthesis* Tumor Necrosis Factor-alpha / genetics Tumor Necrosis Factor-alpha / immunology Tumor Necrosis Factor-alpha / metabolism |
| IF | 4.886 |
| 引用数 | 271 |
| WOS 分野 | IMMUNOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 7 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 遺伝子材料 | hTRAIL/pMKITneo (RDB02934) mTRAIL/pMKITNeo (RDB02935). |