Reference - Detail
|Author||Takechi S, Yamaguchi T, Nomura H, Minematsu T, Adachi M, Kurata H, Kurata R.|
|Title||Mutation spectrum induced by dihydropyrazines in Escherichia coli.|
|Journal||Biol Pharm Bull|
Dihydropyrazine (DHP), which induces mutagenesis in E. coli, was investigated. From analyzing mutations in the chromosomal rpoB gene, the mutation spectrum in uvrB strain revealed the different behavior on exposure to two DHP derivatives 3-hydro-2,2,5,6-tetramethylpyrazine (HTMP), and 2,3-dihydro-5,6-dimethylpyrazine (DHDMP). A higher level of DHP-induced mutation was observed, with base substitutions at G : C pairs predominant. HTMP and DHDMP increased the frequency of G : C to T : A transversions. HTMP increased the frequency of G : C to A : T transitions, than did DHDMP. These findings suggest that DHPs prefer to attack the G : C pair and that different DHP derivatives may prefer distinct mutagenic base pairs; and further, that nucleotide excision repair may be involved in the repair of DHP-induced mutations.
|MeSH||Antibiotics, Antitubercular / pharmacology Bacterial Proteins / genetics DNA Helicases / genetics DNA Mutational Analysis DNA Repair / drug effects DNA, Bacterial / biosynthesis DNA, Bacterial / genetics DNA-Directed RNA Polymerases Drug Resistance, Bacterial / genetics Escherichia coli / drug effects* Escherichia coli / genetics* Escherichia coli Proteins / genetics Mutagenesis Mutagens / chemical synthesis Mutagens / pharmacology* Mutation / drug effects* Pyrazines / pharmacology* Rifampin / pharmacology|
|WOS Category||PHARMACOLOGY & PHARMACY|
|Prokaryotes E. coli||ME8079(AB1157) ME8080(AB1885)|