論文 - 詳細
| RRC ID | 33032 |
|---|---|
| 著者 | Zirin J, Nieuwenhuis J, Samsonova A, Tao R, Perrimon N. |
| タイトル | Regulators of autophagosome formation in Drosophila muscles. |
| ジャーナル | PLoS Genet |
| Abstract |
Given the diversity of autophagy targets and regulation, it is important to characterize autophagy in various cell types and conditions. We used a primary myocyte cell culture system to assay the role of putative autophagy regulators in the specific context of skeletal muscle. By treating the cultures with rapamycin (Rap) and chloroquine (CQ) we induced an autophagic response, fully suppressible by knockdown of core ATG genes. We screened D. melanogaster orthologs of a previously reported mammalian autophagy protein-protein interaction network, identifying several proteins required for autophagosome formation in muscle cells, including orthologs of the Rab regulators RabGap1 and Rab3Gap1. The screen also highlighted the critical roles of the proteasome and glycogen metabolism in regulating autophagy. Specifically, sustained proteasome inhibition inhibited autophagosome formation both in primary culture and larval skeletal muscle, even though autophagy normally acts to suppress ubiquitin aggregate formation in these tissues. In addition, analyses of glycogen metabolic genes in both primary cultured and larval muscles indicated that glycogen storage enhances the autophagic response to starvation, an important insight given the link between glycogen storage disorders, autophagy, and muscle function. |
| 巻・号 | 11(2) |
| ページ | e1005006 |
| 公開日 | 2015-2-1 |
| DOI | 10.1371/journal.pgen.1005006 |
| PII | PGENETICS-D-13-03270 |
| PMID | 25692684 |
| PMC | PMC4334200 |
| MeSH | Animals Autophagy / genetics* Cell Communication / genetics Chloroquine / administration & dosage Drosophila melanogaster GTPase-Activating Proteins / genetics GTPase-Activating Proteins / metabolism Glycogen / biosynthesis Larva Muscle Cells / cytology Muscle Cells / metabolism Muscle, Skeletal / growth & development* Muscle, Skeletal / metabolism Muscular Diseases / etiology Muscular Diseases / metabolism Muscular Diseases / pathology Phagosomes / genetics* Phagosomes / metabolism Phagosomes / pathology Protein Interaction Maps / genetics* Sirolimus / administration & dosage Ubiquitin / genetics rab3 GTP-Binding Proteins / genetics rab3 GTP-Binding Proteins / metabolism |
| IF | 5.175 |
| 引用数 | 11 |
| WOS 分野 | GENETICS & HEREDITY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
|
| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 2 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ショウジョウバエ | 31935R-1 |